Three-dimensional label-free histological imaging of whole organs by microtomy-assisted autofluorescence tomography
Summary: Three-dimensional (3D) histology is vitally important to characterize disease-induced tissue heterogeneity at the individual cell level. However, it remains challenging for both high-throughput 3D imaging and volumetric reconstruction. Here we propose a label-free, cost-effective, and ready...
| প্রধান লেখক: | , , , , |
|---|---|
| বিন্যাস: | প্রবন্ধ |
| ভাষা: | English |
| প্রকাশিত: |
Elsevier
2022-01-01
|
| মালা: | iScience |
| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | http://www.sciencedirect.com/science/article/pii/S2589004221016916 |
| _version_ | 1831704552879947776 |
|---|---|
| author | Yan Zhang Lei Kang Wentao Yu Victor T.C. Tsang Terence T.W. Wong |
| author_facet | Yan Zhang Lei Kang Wentao Yu Victor T.C. Tsang Terence T.W. Wong |
| author_sort | Yan Zhang |
| collection | DOAJ |
| description | Summary: Three-dimensional (3D) histology is vitally important to characterize disease-induced tissue heterogeneity at the individual cell level. However, it remains challenging for both high-throughput 3D imaging and volumetric reconstruction. Here we propose a label-free, cost-effective, and ready-to-use 3D histological imaging technique, termed microtomy-assisted autofluorescence tomography with ultraviolet excitation (MATE). With the combination of block-face imaging and serial microtome sectioning, MATE can achieve rapid and label-free imaging of paraffin-embedded whole organs at an acquisition speed of 1 cm3 per 4 h with a voxel resolution of 1.2 × 1.2 × 10 μm3. We demonstrate that MATE enables simultaneous visualization of cell nuclei, fiber tracts, and blood vessels in mouse/human brains without tissue staining or clearing. Moreover, diagnostic features, including nuclear size and packing density, can be quantitatively extracted with high accuracy. MATE is augmented to the current slide-based 2D histology, holding great promise to facilitate histopathological interpretation at the organelle level. |
| first_indexed | 2024-12-20T16:01:15Z |
| format | Article |
| id | doaj.art-c7e086592ce541c0a8397eb3ad754012 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-20T16:01:15Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-c7e086592ce541c0a8397eb3ad7540122022-12-21T19:34:17ZengElsevieriScience2589-00422022-01-01251103721Three-dimensional label-free histological imaging of whole organs by microtomy-assisted autofluorescence tomographyYan Zhang0Lei Kang1Wentao Yu2Victor T.C. Tsang3Terence T.W. Wong4Translational and Advanced Bioimaging Laboratory, Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, ChinaTranslational and Advanced Bioimaging Laboratory, Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, ChinaTranslational and Advanced Bioimaging Laboratory, Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, ChinaTranslational and Advanced Bioimaging Laboratory, Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, ChinaTranslational and Advanced Bioimaging Laboratory, Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Kowloon, Hong Kong, China; Corresponding authorSummary: Three-dimensional (3D) histology is vitally important to characterize disease-induced tissue heterogeneity at the individual cell level. However, it remains challenging for both high-throughput 3D imaging and volumetric reconstruction. Here we propose a label-free, cost-effective, and ready-to-use 3D histological imaging technique, termed microtomy-assisted autofluorescence tomography with ultraviolet excitation (MATE). With the combination of block-face imaging and serial microtome sectioning, MATE can achieve rapid and label-free imaging of paraffin-embedded whole organs at an acquisition speed of 1 cm3 per 4 h with a voxel resolution of 1.2 × 1.2 × 10 μm3. We demonstrate that MATE enables simultaneous visualization of cell nuclei, fiber tracts, and blood vessels in mouse/human brains without tissue staining or clearing. Moreover, diagnostic features, including nuclear size and packing density, can be quantitatively extracted with high accuracy. MATE is augmented to the current slide-based 2D histology, holding great promise to facilitate histopathological interpretation at the organelle level.http://www.sciencedirect.com/science/article/pii/S2589004221016916Biological sciencesBiophysicsHistologyOptical imaging |
| spellingShingle | Yan Zhang Lei Kang Wentao Yu Victor T.C. Tsang Terence T.W. Wong Three-dimensional label-free histological imaging of whole organs by microtomy-assisted autofluorescence tomography iScience Biological sciences Biophysics Histology Optical imaging |
| title | Three-dimensional label-free histological imaging of whole organs by microtomy-assisted autofluorescence tomography |
| title_full | Three-dimensional label-free histological imaging of whole organs by microtomy-assisted autofluorescence tomography |
| title_fullStr | Three-dimensional label-free histological imaging of whole organs by microtomy-assisted autofluorescence tomography |
| title_full_unstemmed | Three-dimensional label-free histological imaging of whole organs by microtomy-assisted autofluorescence tomography |
| title_short | Three-dimensional label-free histological imaging of whole organs by microtomy-assisted autofluorescence tomography |
| title_sort | three dimensional label free histological imaging of whole organs by microtomy assisted autofluorescence tomography |
| topic | Biological sciences Biophysics Histology Optical imaging |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221016916 |
| work_keys_str_mv | AT yanzhang threedimensionallabelfreehistologicalimagingofwholeorgansbymicrotomyassistedautofluorescencetomography AT leikang threedimensionallabelfreehistologicalimagingofwholeorgansbymicrotomyassistedautofluorescencetomography AT wentaoyu threedimensionallabelfreehistologicalimagingofwholeorgansbymicrotomyassistedautofluorescencetomography AT victortctsang threedimensionallabelfreehistologicalimagingofwholeorgansbymicrotomyassistedautofluorescencetomography AT terencetwwong threedimensionallabelfreehistologicalimagingofwholeorgansbymicrotomyassistedautofluorescencetomography |