Antibody elution with 2-me/SDS solution: Uses for multi-layer immunohistochemical analysis of wholemount preparations of human colonic myenteric plexus

Antibody elution with 2-me/SDS solution: Uses for multi-layer immunohistochemical analysis of wholemount preparations of human colonic myenteric plexus

Indirect immunofluorescence is usually restricted to 3-5 markers per preparation, limiting analysis of coexistence. A solution containing 2-mercaptoethanol and sodium dodecyl sulfate (2-ME/SDS) can elute indirect immunofluorescence labelling (i.e. primary antisera followed by fluorophore-conjugated...

Full description

Bibliographic Details
Main Authors: Adam Humenick, M.E. Johnson, B.N. Chen, M. Wee, D.A. Wattchow, M. Costa, P.G. Dinning, S.J.H. Brookes
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:Heliyon
Subjects:
Fluorescent antibody technique
Indirect
Protein denaturation
Lipofuscin
Myenteric plexus
Enteric nervous system
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844024025532

Internet

http://www.sciencedirect.com/science/article/pii/S2405844024025532

Similar Items