A general strategy for expanding polymerase function by droplet microfluidics
Droplet-based optical polymerase sorting employs a fluorescent sensor to monitor polymerase activity inside the microenvironment of uniform water-in-oil emulsions. Here, the authors use this technique to select and isolate single cells for evolution of an unnatural nucleic acid polymerase.
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2016-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms11235 |