A general strategy for expanding polymerase function by droplet microfluidics
Droplet-based optical polymerase sorting employs a fluorescent sensor to monitor polymerase activity inside the microenvironment of uniform water-in-oil emulsions. Here, the authors use this technique to select and isolate single cells for evolution of an unnatural nucleic acid polymerase.
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2016-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms11235 |
| _version_ | 1818843902079139840 |
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| author | Andrew C. Larsen Matthew R. Dunn Andrew Hatch Sujay P. Sau Cody Youngbull John C. Chaput |
| author_facet | Andrew C. Larsen Matthew R. Dunn Andrew Hatch Sujay P. Sau Cody Youngbull John C. Chaput |
| author_sort | Andrew C. Larsen |
| collection | DOAJ |
| description | Droplet-based optical polymerase sorting employs a fluorescent sensor to monitor polymerase activity inside the microenvironment of uniform water-in-oil emulsions. Here, the authors use this technique to select and isolate single cells for evolution of an unnatural nucleic acid polymerase. |
| first_indexed | 2024-12-19T05:05:14Z |
| format | Article |
| id | doaj.art-c7f8937b5925459eaf6e4d4fd43af4f8 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T05:05:14Z |
| publishDate | 2016-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-c7f8937b5925459eaf6e4d4fd43af4f82022-12-21T20:34:58ZengNature PortfolioNature Communications2041-17232016-04-01711910.1038/ncomms11235A general strategy for expanding polymerase function by droplet microfluidicsAndrew C. Larsen0Matthew R. Dunn1Andrew Hatch2Sujay P. Sau3Cody Youngbull4John C. Chaput5The Biodesign Institute, Arizona State UniversityThe Biodesign Institute, Arizona State UniversitySchool of Earth and Space Exploration, Arizona State UniversityThe Biodesign Institute, Arizona State UniversitySchool of Earth and Space Exploration, Arizona State UniversityThe Biodesign Institute, Arizona State UniversityDroplet-based optical polymerase sorting employs a fluorescent sensor to monitor polymerase activity inside the microenvironment of uniform water-in-oil emulsions. Here, the authors use this technique to select and isolate single cells for evolution of an unnatural nucleic acid polymerase.https://doi.org/10.1038/ncomms11235 |
| spellingShingle | Andrew C. Larsen Matthew R. Dunn Andrew Hatch Sujay P. Sau Cody Youngbull John C. Chaput A general strategy for expanding polymerase function by droplet microfluidics Nature Communications |
| title | A general strategy for expanding polymerase function by droplet microfluidics |
| title_full | A general strategy for expanding polymerase function by droplet microfluidics |
| title_fullStr | A general strategy for expanding polymerase function by droplet microfluidics |
| title_full_unstemmed | A general strategy for expanding polymerase function by droplet microfluidics |
| title_short | A general strategy for expanding polymerase function by droplet microfluidics |
| title_sort | general strategy for expanding polymerase function by droplet microfluidics |
| url | https://doi.org/10.1038/ncomms11235 |
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