Fusing Artificial Cell Compartments and Lipid Domains Using Optical Traps: A Tool to Modulate Membrane Composition and Phase Behaviour
New technologies for manipulating biomembranes have vast potential to aid the understanding of biological phenomena, and as tools to sculpt novel artificial cell architectures for synthetic biology. The manipulation and fusion of vesicles using optical traps is amongst the most promising due to the...
| Główni autorzy: | , , |
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| Format: | Artykuł |
| Język: | English |
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MDPI AG
2020-04-01
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| Seria: | Micromachines |
| Hasła przedmiotowe: | |
| Dostęp online: | https://www.mdpi.com/2072-666X/11/4/388 |
| _version_ | 1827719226650001408 |
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| author | Adithya Vivek Guido Bolognesi Yuval Elani |
| author_facet | Adithya Vivek Guido Bolognesi Yuval Elani |
| author_sort | Adithya Vivek |
| collection | DOAJ |
| description | New technologies for manipulating biomembranes have vast potential to aid the understanding of biological phenomena, and as tools to sculpt novel artificial cell architectures for synthetic biology. The manipulation and fusion of vesicles using optical traps is amongst the most promising due to the level of spatiotemporal control it affords. Herein, we conduct a suite of feasibility studies to show the potential of optical trapping technologies to (i) modulate the lipid composition of a vesicle by delivering new membrane material through fusion events and (ii) manipulate and controllably fuse coexisting membrane domains for the first time. We also outline some noteworthy morphologies and transitions that the vesicle undergoes during fusion, which gives us insight into the mechanisms at play. These results will guide future exploitation of laser-assisted membrane manipulation methods and feed into a technology roadmap for this emerging technology. |
| first_indexed | 2024-03-10T20:38:08Z |
| format | Article |
| id | doaj.art-c82663501a3d4500a1e24b3b7cb8bf8a |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T20:38:08Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-c82663501a3d4500a1e24b3b7cb8bf8a2023-11-19T20:54:20ZengMDPI AGMicromachines2072-666X2020-04-0111438810.3390/mi11040388Fusing Artificial Cell Compartments and Lipid Domains Using Optical Traps: A Tool to Modulate Membrane Composition and Phase BehaviourAdithya Vivek0Guido Bolognesi1Yuval Elani2Department of Chemistry, Imperial College London, London W12 0BZ, UKDepartment of Chemical Engineering, Loughborough University, Leicestershire LE11 3TU, UKDepartment of Chemical Engineering, Imperial College London, London SW7 2AZ, UKNew technologies for manipulating biomembranes have vast potential to aid the understanding of biological phenomena, and as tools to sculpt novel artificial cell architectures for synthetic biology. The manipulation and fusion of vesicles using optical traps is amongst the most promising due to the level of spatiotemporal control it affords. Herein, we conduct a suite of feasibility studies to show the potential of optical trapping technologies to (i) modulate the lipid composition of a vesicle by delivering new membrane material through fusion events and (ii) manipulate and controllably fuse coexisting membrane domains for the first time. We also outline some noteworthy morphologies and transitions that the vesicle undergoes during fusion, which gives us insight into the mechanisms at play. These results will guide future exploitation of laser-assisted membrane manipulation methods and feed into a technology roadmap for this emerging technology.https://www.mdpi.com/2072-666X/11/4/388optical trapsvesiclesartificial cellsmembrane biophysicsphase separationmembranes |
| spellingShingle | Adithya Vivek Guido Bolognesi Yuval Elani Fusing Artificial Cell Compartments and Lipid Domains Using Optical Traps: A Tool to Modulate Membrane Composition and Phase Behaviour Micromachines optical traps vesicles artificial cells membrane biophysics phase separation membranes |
| title | Fusing Artificial Cell Compartments and Lipid Domains Using Optical Traps: A Tool to Modulate Membrane Composition and Phase Behaviour |
| title_full | Fusing Artificial Cell Compartments and Lipid Domains Using Optical Traps: A Tool to Modulate Membrane Composition and Phase Behaviour |
| title_fullStr | Fusing Artificial Cell Compartments and Lipid Domains Using Optical Traps: A Tool to Modulate Membrane Composition and Phase Behaviour |
| title_full_unstemmed | Fusing Artificial Cell Compartments and Lipid Domains Using Optical Traps: A Tool to Modulate Membrane Composition and Phase Behaviour |
| title_short | Fusing Artificial Cell Compartments and Lipid Domains Using Optical Traps: A Tool to Modulate Membrane Composition and Phase Behaviour |
| title_sort | fusing artificial cell compartments and lipid domains using optical traps a tool to modulate membrane composition and phase behaviour |
| topic | optical traps vesicles artificial cells membrane biophysics phase separation membranes |
| url | https://www.mdpi.com/2072-666X/11/4/388 |
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