Generation of human elongating multi-lineage organized cardiac gastruloids
Summary: Human elongating multi-lineage organized (EMLOC) gastruloid technology captures key aspects of trunk neurodevelopment including neural integration with cardiogenesis. We generate multi-chambered, contractile EMLOC gastruloids with integrated central and peripheral neurons using defined cult...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672200778X |
| _version_ | 1811292138738024448 |
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| author | Zachary T. Olmsted Maria Belen Paredes-Espinosa Janet L. Paluh |
| author_facet | Zachary T. Olmsted Maria Belen Paredes-Espinosa Janet L. Paluh |
| author_sort | Zachary T. Olmsted |
| collection | DOAJ |
| description | Summary: Human elongating multi-lineage organized (EMLOC) gastruloid technology captures key aspects of trunk neurodevelopment including neural integration with cardiogenesis. We generate multi-chambered, contractile EMLOC gastruloids with integrated central and peripheral neurons using defined culture conditions and signaling factors. hiPSC colonies are primed by activating FGF and Wnt signaling pathways for co-induced lineages. EMLOC gastruloids are then initialized with primed cells in suspension culture using timed exposure to FGF2, HGF, IGF1, and Y-27632. Cardiogenesis is stimulated by FGF2, VEGF, and ascorbic acid.For complete details on the use and execution of this protocol, please refer to Olmsted and Paluh (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-13T04:40:46Z |
| format | Article |
| id | doaj.art-c837673abfcc439dae2398d702c15855 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T04:40:46Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-c837673abfcc439dae2398d702c158552022-12-22T03:02:00ZengElsevierSTAR Protocols2666-16672022-12-0134101898Generation of human elongating multi-lineage organized cardiac gastruloidsZachary T. Olmsted0Maria Belen Paredes-Espinosa1Janet L. Paluh2State University of New York Polytechnic Institute, College of Nanoscale Science and Engineering, Nanobioscience, Albany, NY 12203, USA; ZTO at State University of New York Downstate Medical Center, New York, NY, USAState University of New York Polytechnic Institute, College of Nanoscale Science and Engineering, Nanobioscience, Albany, NY 12203, USAState University of New York Polytechnic Institute, College of Nanoscale Science and Engineering, Nanobioscience, Albany, NY 12203, USA; Corresponding authorSummary: Human elongating multi-lineage organized (EMLOC) gastruloid technology captures key aspects of trunk neurodevelopment including neural integration with cardiogenesis. We generate multi-chambered, contractile EMLOC gastruloids with integrated central and peripheral neurons using defined culture conditions and signaling factors. hiPSC colonies are primed by activating FGF and Wnt signaling pathways for co-induced lineages. EMLOC gastruloids are then initialized with primed cells in suspension culture using timed exposure to FGF2, HGF, IGF1, and Y-27632. Cardiogenesis is stimulated by FGF2, VEGF, and ascorbic acid.For complete details on the use and execution of this protocol, please refer to Olmsted and Paluh (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S266616672200778XBioinformaticsDevelopmental biologyNeuroscienceStem CellsOrganoids |
| spellingShingle | Zachary T. Olmsted Maria Belen Paredes-Espinosa Janet L. Paluh Generation of human elongating multi-lineage organized cardiac gastruloids STAR Protocols Bioinformatics Developmental biology Neuroscience Stem Cells Organoids |
| title | Generation of human elongating multi-lineage organized cardiac gastruloids |
| title_full | Generation of human elongating multi-lineage organized cardiac gastruloids |
| title_fullStr | Generation of human elongating multi-lineage organized cardiac gastruloids |
| title_full_unstemmed | Generation of human elongating multi-lineage organized cardiac gastruloids |
| title_short | Generation of human elongating multi-lineage organized cardiac gastruloids |
| title_sort | generation of human elongating multi lineage organized cardiac gastruloids |
| topic | Bioinformatics Developmental biology Neuroscience Stem Cells Organoids |
| url | http://www.sciencedirect.com/science/article/pii/S266616672200778X |
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