Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits
A comprehensive characterization of the phytochemicals present in a blackberry fruit extract by HPLC-TOF-MS has been carried out. The main compounds in the extract were ursane-type terpenoids which, along with phenolic compounds, may be responsible for the bioactivity of the extract. In vitro antiox...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/7/1505 |
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| author | Lidia Gil-Martínez Nuria Mut-Salud José Antonio Ruiz-García Ana Falcón-Piñeiro Mònica Maijó-Ferré Alberto Baños José Manuel De la Torre-Ramírez Enrique Guillamón Vito Verardo Ana María Gómez-Caravaca |
| author_facet | Lidia Gil-Martínez Nuria Mut-Salud José Antonio Ruiz-García Ana Falcón-Piñeiro Mònica Maijó-Ferré Alberto Baños José Manuel De la Torre-Ramírez Enrique Guillamón Vito Verardo Ana María Gómez-Caravaca |
| author_sort | Lidia Gil-Martínez |
| collection | DOAJ |
| description | A comprehensive characterization of the phytochemicals present in a blackberry fruit extract by HPLC-TOF-MS has been carried out. The main compounds in the extract were ursane-type terpenoids which, along with phenolic compounds, may be responsible for the bioactivity of the extract. In vitro antioxidant capacity was assessed through Folin–Ciocalteu (31.05 ± 4.9 mg GAE/g d.w.), FRAP (637.8 ± 3.2 μmol Fe<sup>2+</sup>/g d.w.), DPPH (IC<sub>50</sub> 97.1 ± 2.4 μg d.w./mL) and TEAC (576.6 ± 8.3 μmol TE/g d.w.) assays. Furthermore, the extract exerted remarkable effects on in vitro cellular antioxidant activity in HUVEC cells at a concentration of 5 mg/mL. Antimicrobial activity of the extract was also tested. Most sensible microorganisms were Gram-positive bacteria, such as <i>E. faecalis</i>, <i>B. cereus</i> and Gram-negative <i>E. coli</i> (MBC of 12.5 mg/mL). IC<sub>50</sub> values against colon tumoral cells HT-29 (4.9 ± 0.2 mg/mL), T-84 (5.9 ± 0.3 mg/mL) and SW-837 (5.9 ± 0.2 mg/mL) were also obtained. Furthermore, blackberry extract demonstrated anti-inflammatory activity inhibiting the secretion of pro-inflammatory IL-8 cytokines in two cellular models (HT-29 and T-84) in a concentration-dependent manner. These results support that blackberry fruits are an interesting source of bioactive compounds that may be useful in the prevention and treatment of different diseases, mainly related to oxidative stress. |
| first_indexed | 2024-03-11T05:37:11Z |
| format | Article |
| id | doaj.art-c843ca7acf0e46d2b74dc5952d5f9df5 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T05:37:11Z |
| publishDate | 2023-04-01 |
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| series | Foods |
| spelling | doaj.art-c843ca7acf0e46d2b74dc5952d5f9df52023-11-17T16:42:13ZengMDPI AGFoods2304-81582023-04-01127150510.3390/foods12071505Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry FruitsLidia Gil-Martínez0Nuria Mut-Salud1José Antonio Ruiz-García2Ana Falcón-Piñeiro3Mònica Maijó-Ferré4Alberto Baños5José Manuel De la Torre-Ramírez6Enrique Guillamón7Vito Verardo8Ana María Gómez-Caravaca9Department of Analytical Chemistry, University of Granada, Avda Fuentenueva, 18071 Granada, SpainDepartment of Microbiology, University of Granada, Avda Fuentenueva, 18071 Granada, SpainDepartment of Nutrition and Food Science, University of Granada, Campus of Cartuja, 18071 Granada, SpainDepartment of Microbiology, University of Granada, Avda Fuentenueva, 18071 Granada, SpainLeitat Technological Center, Carrer Innovació, 2, 08225 Terrassa, SpainDepartment of Microbiology, University of Granada, Avda Fuentenueva, 18071 Granada, SpainDepartment of Organic and Inorganic Chemistry, University of Jaen, 27071 Jaen, SpainDepartment of Chemical Engineering, University of Granada, Avda Fuentenueva, 18071 Granada, SpainDepartment of Nutrition and Food Science, University of Granada, Campus of Cartuja, 18071 Granada, SpainDepartment of Analytical Chemistry, University of Granada, Avda Fuentenueva, 18071 Granada, SpainA comprehensive characterization of the phytochemicals present in a blackberry fruit extract by HPLC-TOF-MS has been carried out. The main compounds in the extract were ursane-type terpenoids which, along with phenolic compounds, may be responsible for the bioactivity of the extract. In vitro antioxidant capacity was assessed through Folin–Ciocalteu (31.05 ± 4.9 mg GAE/g d.w.), FRAP (637.8 ± 3.2 μmol Fe<sup>2+</sup>/g d.w.), DPPH (IC<sub>50</sub> 97.1 ± 2.4 μg d.w./mL) and TEAC (576.6 ± 8.3 μmol TE/g d.w.) assays. Furthermore, the extract exerted remarkable effects on in vitro cellular antioxidant activity in HUVEC cells at a concentration of 5 mg/mL. Antimicrobial activity of the extract was also tested. Most sensible microorganisms were Gram-positive bacteria, such as <i>E. faecalis</i>, <i>B. cereus</i> and Gram-negative <i>E. coli</i> (MBC of 12.5 mg/mL). IC<sub>50</sub> values against colon tumoral cells HT-29 (4.9 ± 0.2 mg/mL), T-84 (5.9 ± 0.3 mg/mL) and SW-837 (5.9 ± 0.2 mg/mL) were also obtained. Furthermore, blackberry extract demonstrated anti-inflammatory activity inhibiting the secretion of pro-inflammatory IL-8 cytokines in two cellular models (HT-29 and T-84) in a concentration-dependent manner. These results support that blackberry fruits are an interesting source of bioactive compounds that may be useful in the prevention and treatment of different diseases, mainly related to oxidative stress.https://www.mdpi.com/2304-8158/12/7/1505<i>Rubus fruticosus</i>phenolic compoundsterpenic compoundsbiological activityHPLC-MS |
| spellingShingle | Lidia Gil-Martínez Nuria Mut-Salud José Antonio Ruiz-García Ana Falcón-Piñeiro Mònica Maijó-Ferré Alberto Baños José Manuel De la Torre-Ramírez Enrique Guillamón Vito Verardo Ana María Gómez-Caravaca Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits Foods <i>Rubus fruticosus</i> phenolic compounds terpenic compounds biological activity HPLC-MS |
| title | Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits |
| title_full | Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits |
| title_fullStr | Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits |
| title_full_unstemmed | Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits |
| title_short | Phytochemicals Determination, and Antioxidant, Antimicrobial, Anti-Inflammatory and Anticancer Activities of Blackberry Fruits |
| title_sort | phytochemicals determination and antioxidant antimicrobial anti inflammatory and anticancer activities of blackberry fruits |
| topic | <i>Rubus fruticosus</i> phenolic compounds terpenic compounds biological activity HPLC-MS |
| url | https://www.mdpi.com/2304-8158/12/7/1505 |
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