Withaferin A inhibits osteosarcoma cells through inactivation of Notch-1 signalling
Human osteosarcoma cells were treated with withaferin A and the antiproliferative activity was evaluated by the 3-(4, 5 dimethyl-thiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays. Real-time RT-PCR and western blot were used to investigate whether the down-regulation of Notch-1 contributes...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bangladesh Pharmacological Society
2014-08-01
|
| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/19568 |
| _version_ | 1818551676642000896 |
|---|---|
| author | Yang Chen Xiang-Zhen Han Wei Wang Ren-Tao Zhao Xiao Li |
| author_facet | Yang Chen Xiang-Zhen Han Wei Wang Ren-Tao Zhao Xiao Li |
| author_sort | Yang Chen |
| collection | DOAJ |
| description | Human osteosarcoma cells were treated with withaferin A and the antiproliferative activity was evaluated by the 3-(4, 5 dimethyl-thiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays. Real-time RT-PCR and western blot were used to investigate whether the down-regulation of Notch-1 contributes to withaferin A-induced inhibition of proliferation in osteosar-coma cells. The results showed that withaferin A caused marked inhibition of osteosarcoma cell growth and G2?M phase cell cycle arrest. This was associated with concomitant attenuation of Notch-1 and down-regulation of its downstream genes, such as matrix metalloproteinases. These results suggest that antitumor activity of withaferin A is mediated through a novel mechanism involving inactivation of the Notch-1 signalling pathway. Our data provide the first evidence that the down regulation of Notch-1 by withaferin A may be an effective approach for the treatment of osteosarcoma. |
| first_indexed | 2024-12-12T09:03:04Z |
| format | Article |
| id | doaj.art-c85402eac8874a0fa5edb4148ac639bd |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-12-12T09:03:04Z |
| publishDate | 2014-08-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-c85402eac8874a0fa5edb4148ac639bd2022-12-22T00:29:47ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882014-08-019310.3329/bjp.v9i3.19568Withaferin A inhibits osteosarcoma cells through inactivation of Notch-1 signallingYang Chen0Xiang-Zhen Han1Wei Wang2Ren-Tao Zhao3Xiao Li4Department of Bone Tumor, Linyi People’s Hospital, Shandong 276 003Department of Bone Tumor, Linyi People’s Hospital, Shandong 276 003Department of Bone Tumor, Linyi People’s Hospital, Shandong 276 003Department of Bone Tumor, Linyi People’s Hospital, Shandong 276 003Department of Bone Tumor, Linyi People’s Hospital, Shandong 276 003Human osteosarcoma cells were treated with withaferin A and the antiproliferative activity was evaluated by the 3-(4, 5 dimethyl-thiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays. Real-time RT-PCR and western blot were used to investigate whether the down-regulation of Notch-1 contributes to withaferin A-induced inhibition of proliferation in osteosar-coma cells. The results showed that withaferin A caused marked inhibition of osteosarcoma cell growth and G2?M phase cell cycle arrest. This was associated with concomitant attenuation of Notch-1 and down-regulation of its downstream genes, such as matrix metalloproteinases. These results suggest that antitumor activity of withaferin A is mediated through a novel mechanism involving inactivation of the Notch-1 signalling pathway. Our data provide the first evidence that the down regulation of Notch-1 by withaferin A may be an effective approach for the treatment of osteosarcoma.https://www.banglajol.info/index.php/BJP/article/view/19568Withaferin AWithanolidesAdenocarcinomaPhosphorylationApoptosis |
| spellingShingle | Yang Chen Xiang-Zhen Han Wei Wang Ren-Tao Zhao Xiao Li Withaferin A inhibits osteosarcoma cells through inactivation of Notch-1 signalling Bangladesh Journal of Pharmacology Withaferin A Withanolides Adenocarcinoma Phosphorylation Apoptosis |
| title | Withaferin A inhibits osteosarcoma cells through inactivation of Notch-1 signalling |
| title_full | Withaferin A inhibits osteosarcoma cells through inactivation of Notch-1 signalling |
| title_fullStr | Withaferin A inhibits osteosarcoma cells through inactivation of Notch-1 signalling |
| title_full_unstemmed | Withaferin A inhibits osteosarcoma cells through inactivation of Notch-1 signalling |
| title_short | Withaferin A inhibits osteosarcoma cells through inactivation of Notch-1 signalling |
| title_sort | withaferin a inhibits osteosarcoma cells through inactivation of notch 1 signalling |
| topic | Withaferin A Withanolides Adenocarcinoma Phosphorylation Apoptosis |
| url | https://www.banglajol.info/index.php/BJP/article/view/19568 |
| work_keys_str_mv | AT yangchen withaferinainhibitsosteosarcomacellsthroughinactivationofnotch1signalling AT xiangzhenhan withaferinainhibitsosteosarcomacellsthroughinactivationofnotch1signalling AT weiwang withaferinainhibitsosteosarcomacellsthroughinactivationofnotch1signalling AT rentaozhao withaferinainhibitsosteosarcomacellsthroughinactivationofnotch1signalling AT xiaoli withaferinainhibitsosteosarcomacellsthroughinactivationofnotch1signalling |