Optimization and Identification of Single Mutation in Hemoglobin Variants with 2,2,2 Trifluoroethanol Modified Digestion Method and Nano−LC Coupled MALDI MS/MS

Optimization and Identification of Single Mutation in Hemoglobin Variants with 2,2,2 Trifluoroethanol Modified Digestion Method and Nano−LC Coupled MALDI MS/MS

<b>Background:</b> Hemoglobin (Hb) variants arise due to point mutations in globin chains and their pathological treatments rely heavily on the identification of the nature and location of the mutation in the globin chains. Traditional methods for diagnosis such as HPLC and electrophores...

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Bibliographic Details
Main Authors: Pushpanjali Dasauni, Nirpendra Singh, Varun Chhabra, Manoranjan Mahapatra, Renu Saxena, Suman Kundu
Format: Article
Language:English
Published: MDPI AG 2022-09-01
Series:Molecules
Subjects:
hemoglobin disorders
mass spectrometry
hemoglobin variants
liquid chromatography
MALDI TOF/TOF
nano LC−MALDI MS/MS
Online Access:https://www.mdpi.com/1420-3049/27/19/6357

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https://www.mdpi.com/1420-3049/27/19/6357

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