Allele-Specific PCR for PIK3CA Mutation Detection Using Phosphoryl Guanidine Modified Primers

Allele-Specific PCR for PIK3CA Mutation Detection Using Phosphoryl Guanidine Modified Primers

Phosphoryl guanidine (PG) is the novel uncharged modification of internucleotide phosphates of oligonucleotides. Incorporating PG modification into PCR primers leads to increased discrimination between wild-type and mutated DNA, providing extraordinary detection limits in an allele-specific real-tim...

Full description

Bibliographic Details
Main Authors: Alexey S. Chubarov, Igor P. Oscorbin, Lidiya M. Novikova, Maxim L. Filipenko, Alexander A. Lomzov, Dmitrii V. Pyshnyi
Format: Article
Language:English
Published: MDPI AG 2023-01-01
Series:Diagnostics
Subjects:
mutation detection
PIK3CA mutations
allele-specific PCR
modified oligonucleotides
phosphoryl guanidine oligonucleotide
Online Access:https://www.mdpi.com/2075-4418/13/2/250

Internet

https://www.mdpi.com/2075-4418/13/2/250

Similar Items