SFRP4+IGFBP5hi NKT cells induced neural-like cell differentiation to contribute to adenomyosis pain
BackgroundAdenomyosis is an estrogen-dependent gynecological disease. The pathogenesis of chronic pain, the main clinical symptom of adenomyosis, remains undefined. As a combination lymphocyte with both T-cell and natural killer (NK)–cell properties, NK T (NKT) cells play a role in immune defense ag...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.945504/full |
| _version_ | 1811213853991632896 |
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| author | Yichen Chen Jue Zhu Liang Chen Yuanyuan Shen Jing Zhang Qiming Wang |
| author_facet | Yichen Chen Jue Zhu Liang Chen Yuanyuan Shen Jing Zhang Qiming Wang |
| author_sort | Yichen Chen |
| collection | DOAJ |
| description | BackgroundAdenomyosis is an estrogen-dependent gynecological disease. The pathogenesis of chronic pain, the main clinical symptom of adenomyosis, remains undefined. As a combination lymphocyte with both T-cell and natural killer (NK)–cell properties, NK T (NKT) cells play a role in immune defense against numerous diseases and modulate cell differentiation.MethodThis study analyzed the tissue-cell samples from adenomyosis with or without pain by single-cell sequencing.ResultWe found a specific population of secreted frizzled-related protein 4 (SFRP4)+NKT cells and a large amount of undifferentiated multipotent stem cells in the adenomyosis pain group. We discovered that a high expression of IGFBP5 in SFRP4+NKT cells could promote the differentiation of multipotent stem cells into neural-like cells via the single-cell trajectory. Through verification by the sample, we found that the degree of the expression of the neuronal marker NEFM was correlated with the duration of pain in adenomyosis patients. The expression of IGFBP5 was positively correlated with the pain scores of adenomyosis patients.ConclusionCollectively, these findings suggest that SFRP4+IGFBP5hi NKT cells were capable of converting part of the stem cells into neurogenic cells and inducing adenomyosis pain. |
| first_indexed | 2024-04-12T05:53:14Z |
| format | Article |
| id | doaj.art-c87e2c611f2c477990a229894ab2366b |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-12T05:53:14Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-c87e2c611f2c477990a229894ab2366b2022-12-22T03:45:15ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-11-011310.3389/fimmu.2022.945504945504SFRP4+IGFBP5hi NKT cells induced neural-like cell differentiation to contribute to adenomyosis painYichen ChenJue ZhuLiang ChenYuanyuan ShenJing ZhangQiming WangBackgroundAdenomyosis is an estrogen-dependent gynecological disease. The pathogenesis of chronic pain, the main clinical symptom of adenomyosis, remains undefined. As a combination lymphocyte with both T-cell and natural killer (NK)–cell properties, NK T (NKT) cells play a role in immune defense against numerous diseases and modulate cell differentiation.MethodThis study analyzed the tissue-cell samples from adenomyosis with or without pain by single-cell sequencing.ResultWe found a specific population of secreted frizzled-related protein 4 (SFRP4)+NKT cells and a large amount of undifferentiated multipotent stem cells in the adenomyosis pain group. We discovered that a high expression of IGFBP5 in SFRP4+NKT cells could promote the differentiation of multipotent stem cells into neural-like cells via the single-cell trajectory. Through verification by the sample, we found that the degree of the expression of the neuronal marker NEFM was correlated with the duration of pain in adenomyosis patients. The expression of IGFBP5 was positively correlated with the pain scores of adenomyosis patients.ConclusionCollectively, these findings suggest that SFRP4+IGFBP5hi NKT cells were capable of converting part of the stem cells into neurogenic cells and inducing adenomyosis pain.https://www.frontiersin.org/articles/10.3389/fimmu.2022.945504/fulladenomyosis painsingle-cell sequencingneural-like cellsNKT cellsIGFBP5SFRP4 |
| spellingShingle | Yichen Chen Jue Zhu Liang Chen Yuanyuan Shen Jing Zhang Qiming Wang SFRP4+IGFBP5hi NKT cells induced neural-like cell differentiation to contribute to adenomyosis pain Frontiers in Immunology adenomyosis pain single-cell sequencing neural-like cells NKT cells IGFBP5 SFRP4 |
| title | SFRP4+IGFBP5hi NKT cells induced neural-like cell differentiation to contribute to adenomyosis pain |
| title_full | SFRP4+IGFBP5hi NKT cells induced neural-like cell differentiation to contribute to adenomyosis pain |
| title_fullStr | SFRP4+IGFBP5hi NKT cells induced neural-like cell differentiation to contribute to adenomyosis pain |
| title_full_unstemmed | SFRP4+IGFBP5hi NKT cells induced neural-like cell differentiation to contribute to adenomyosis pain |
| title_short | SFRP4+IGFBP5hi NKT cells induced neural-like cell differentiation to contribute to adenomyosis pain |
| title_sort | sfrp4 igfbp5hi nkt cells induced neural like cell differentiation to contribute to adenomyosis pain |
| topic | adenomyosis pain single-cell sequencing neural-like cells NKT cells IGFBP5 SFRP4 |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.945504/full |
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