Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins
To mitigate the GFP-triggered artifacts in Fluorescence-detection Size-Exclusion Chromatography (FSEC), Jin et al. develop an FSEC assay utilizing nanobody technology which allows evaluation of the expression, monodispersity and thermostability of membrane proteins without the need for purification....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-03-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-021-01891-y |