Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins
To mitigate the GFP-triggered artifacts in Fluorescence-detection Size-Exclusion Chromatography (FSEC), Jin et al. develop an FSEC assay utilizing nanobody technology which allows evaluation of the expression, monodispersity and thermostability of membrane proteins without the need for purification....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-03-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-021-01891-y |
| _version_ | 1830287301462196224 |
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| author | Fei Jin Cheng Shen Yao Wang Mengqi Wang Minxuan Sun Motoyuki Hattori |
| author_facet | Fei Jin Cheng Shen Yao Wang Mengqi Wang Minxuan Sun Motoyuki Hattori |
| author_sort | Fei Jin |
| collection | DOAJ |
| description | To mitigate the GFP-triggered artifacts in Fluorescence-detection Size-Exclusion Chromatography (FSEC), Jin et al. develop an FSEC assay utilizing nanobody technology which allows evaluation of the expression, monodispersity and thermostability of membrane proteins without the need for purification. It holds promise as a powerful tool for screening of membrane proteins for functional and structural studies. |
| first_indexed | 2024-12-19T04:15:51Z |
| format | Article |
| id | doaj.art-c89bae6b37bc44658ad0cfb2e371e704 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-12-19T04:15:51Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-c89bae6b37bc44658ad0cfb2e371e7042022-12-21T20:36:18ZengNature PortfolioCommunications Biology2399-36422021-03-014111410.1038/s42003-021-01891-yFluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteinsFei Jin0Cheng Shen1Yao Wang2Mengqi Wang3Minxuan Sun4Motoyuki Hattori5State Key Laboratory of Genetic Engineering, Shanghai Key Laboratory of Bioactive Small Molecules, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan UniversityState Key Laboratory of Genetic Engineering, Shanghai Key Laboratory of Bioactive Small Molecules, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan UniversityState Key Laboratory of Genetic Engineering, Shanghai Key Laboratory of Bioactive Small Molecules, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan UniversityState Key Laboratory of Genetic Engineering, Shanghai Key Laboratory of Bioactive Small Molecules, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan UniversityState Key Laboratory of Genetic Engineering, Shanghai Key Laboratory of Bioactive Small Molecules, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan UniversityState Key Laboratory of Genetic Engineering, Shanghai Key Laboratory of Bioactive Small Molecules, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Biophysics, School of Life Sciences, Fudan UniversityTo mitigate the GFP-triggered artifacts in Fluorescence-detection Size-Exclusion Chromatography (FSEC), Jin et al. develop an FSEC assay utilizing nanobody technology which allows evaluation of the expression, monodispersity and thermostability of membrane proteins without the need for purification. It holds promise as a powerful tool for screening of membrane proteins for functional and structural studies.https://doi.org/10.1038/s42003-021-01891-y |
| spellingShingle | Fei Jin Cheng Shen Yao Wang Mengqi Wang Minxuan Sun Motoyuki Hattori Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins Communications Biology |
| title | Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins |
| title_full | Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins |
| title_fullStr | Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins |
| title_full_unstemmed | Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins |
| title_short | Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins |
| title_sort | fluorescence detection size exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins |
| url | https://doi.org/10.1038/s42003-021-01891-y |
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