Metabolic Engineering of <i>Bacillus subtilis</i> for Riboflavin Production: A Review

Riboflavin (vitamin B<sub>2</sub>) is one of the essential vitamins that the human body needs to maintain normal metabolism. Its biosynthesis has become one of the successful models for gradual replacement of traditional chemical production routes. <i>B. subtilis</i> is chara...

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Main Authors: Yang Liu, Quan Zhang, Xiaoxiao Qi, Huipeng Gao, Meng Wang, Hao Guan, Bo Yu
Format: Article
Language:English
Published: MDPI AG 2023-01-01
Series:Microorganisms
Subjects:
Online Access:https://www.mdpi.com/2076-2607/11/1/164
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author Yang Liu
Quan Zhang
Xiaoxiao Qi
Huipeng Gao
Meng Wang
Hao Guan
Bo Yu
author_facet Yang Liu
Quan Zhang
Xiaoxiao Qi
Huipeng Gao
Meng Wang
Hao Guan
Bo Yu
author_sort Yang Liu
collection DOAJ
description Riboflavin (vitamin B<sub>2</sub>) is one of the essential vitamins that the human body needs to maintain normal metabolism. Its biosynthesis has become one of the successful models for gradual replacement of traditional chemical production routes. <i>B. subtilis</i> is characterized by its short fermentation time and high yield, which shows a huge competitive advantage in microbial fermentation for production of riboflavin. This review summarized the advancements of regulation on riboflavin production as well as the synthesis of two precursors of ribulose-5-phosphate riboflavin (Ru5P) and guanosine 5′-triphosphate (GTP) in <i>B. subtilis</i>. The different strategies to improve production of riboflavin by metabolic engineering were also reviewed.
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spelling doaj.art-c89bfb9ca7384c7c97b5880164d64d902023-11-30T23:37:22ZengMDPI AGMicroorganisms2076-26072023-01-0111116410.3390/microorganisms11010164Metabolic Engineering of <i>Bacillus subtilis</i> for Riboflavin Production: A ReviewYang Liu0Quan Zhang1Xiaoxiao Qi2Huipeng Gao3Meng Wang4Hao Guan5Bo Yu6Key Laboratory of Biofuels and Biochemical Engineering, SINOPEC (Dalian) Research Institute of Petroleum and Petro-Chemicals Co., Ltd., Dalian 116045, ChinaKey Laboratory of Biofuels and Biochemical Engineering, SINOPEC (Dalian) Research Institute of Petroleum and Petro-Chemicals Co., Ltd., Dalian 116045, ChinaCAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaKey Laboratory of Biofuels and Biochemical Engineering, SINOPEC (Dalian) Research Institute of Petroleum and Petro-Chemicals Co., Ltd., Dalian 116045, ChinaKey Laboratory of Biofuels and Biochemical Engineering, SINOPEC (Dalian) Research Institute of Petroleum and Petro-Chemicals Co., Ltd., Dalian 116045, ChinaKey Laboratory of Biofuels and Biochemical Engineering, SINOPEC (Dalian) Research Institute of Petroleum and Petro-Chemicals Co., Ltd., Dalian 116045, ChinaCAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, ChinaRiboflavin (vitamin B<sub>2</sub>) is one of the essential vitamins that the human body needs to maintain normal metabolism. Its biosynthesis has become one of the successful models for gradual replacement of traditional chemical production routes. <i>B. subtilis</i> is characterized by its short fermentation time and high yield, which shows a huge competitive advantage in microbial fermentation for production of riboflavin. This review summarized the advancements of regulation on riboflavin production as well as the synthesis of two precursors of ribulose-5-phosphate riboflavin (Ru5P) and guanosine 5′-triphosphate (GTP) in <i>B. subtilis</i>. The different strategies to improve production of riboflavin by metabolic engineering were also reviewed.https://www.mdpi.com/2076-2607/11/1/164riboflavinbiosynthesis<i>Bacillus subtilis</i>metabolic engineering
spellingShingle Yang Liu
Quan Zhang
Xiaoxiao Qi
Huipeng Gao
Meng Wang
Hao Guan
Bo Yu
Metabolic Engineering of <i>Bacillus subtilis</i> for Riboflavin Production: A Review
Microorganisms
riboflavin
biosynthesis
<i>Bacillus subtilis</i>
metabolic engineering
title Metabolic Engineering of <i>Bacillus subtilis</i> for Riboflavin Production: A Review
title_full Metabolic Engineering of <i>Bacillus subtilis</i> for Riboflavin Production: A Review
title_fullStr Metabolic Engineering of <i>Bacillus subtilis</i> for Riboflavin Production: A Review
title_full_unstemmed Metabolic Engineering of <i>Bacillus subtilis</i> for Riboflavin Production: A Review
title_short Metabolic Engineering of <i>Bacillus subtilis</i> for Riboflavin Production: A Review
title_sort metabolic engineering of i bacillus subtilis i for riboflavin production a review
topic riboflavin
biosynthesis
<i>Bacillus subtilis</i>
metabolic engineering
url https://www.mdpi.com/2076-2607/11/1/164
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AT huipenggao metabolicengineeringofibacillussubtilisiforriboflavinproductionareview
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