Increased platelet reactivity in idiopathic pulmonary fibrosis is mediated by a plasma factor.
Idiopathic Pulmonary Fibrosis (IPF) is a progressive, incurable fibrotic interstitial lung disease with a prognosis worse than many cancers. Its pathogenesis is poorly understood. Activated platelets can release pro-fibrotic mediators that have the potential to contribute to lung fibrosis. We determ...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4206466?pdf=render |
| _version_ | 1828387284082229248 |
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| author | Michael G Crooks Ahmed Fahim Khalid M Naseem Alyn H Morice Simon P Hart |
| author_facet | Michael G Crooks Ahmed Fahim Khalid M Naseem Alyn H Morice Simon P Hart |
| author_sort | Michael G Crooks |
| collection | DOAJ |
| description | Idiopathic Pulmonary Fibrosis (IPF) is a progressive, incurable fibrotic interstitial lung disease with a prognosis worse than many cancers. Its pathogenesis is poorly understood. Activated platelets can release pro-fibrotic mediators that have the potential to contribute to lung fibrosis. We determine platelet reactivity in subjects with IPF compared to age-matched controls.Whole blood flow cytometry was used to measure platelet-monocyte aggregate formation, platelet P-selectin expression and platelet fibrinogen binding at basal levels and following stimulation with platelet agonists. A plasma swap approach was used to assess the effect of IPF plasma on control platelets.Subjects with IPF showed greater platelet reactivity than controls. Platelet P-selectin expression was significantly greater in IPF patients than controls following stimulation with 0.1 µM ADP (1.9% positive ±0.5 (mean ± SEM) versus 0.7%±0.1; p = 0.03), 1 µM ADP (9.8%±1.3 versus 3.3%±0.8; p<0.01) and 10 µM ADP (41.3%±4.2 versus 22.5%±2.6; p<0.01). Platelet fibrinogen binding was also increased, and platelet activation resulted in increased platelet-monocyte aggregate formation in IPF patients. Re-suspension of control platelets in plasma taken from subjects with IPF resulted in increased platelet activation compared to control plasma.IPF patients exhibit increased platelet reactivity compared with controls. This hyperactivity may result from the plasma environment since control platelets exhibit increased activation when exposed to IPF plasma. |
| first_indexed | 2024-12-10T05:51:32Z |
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| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T05:51:32Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-c8a6ad66bdf8487bbf26b17c5a97a3cd2022-12-22T02:00:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e11134710.1371/journal.pone.0111347Increased platelet reactivity in idiopathic pulmonary fibrosis is mediated by a plasma factor.Michael G CrooksAhmed FahimKhalid M NaseemAlyn H MoriceSimon P HartIdiopathic Pulmonary Fibrosis (IPF) is a progressive, incurable fibrotic interstitial lung disease with a prognosis worse than many cancers. Its pathogenesis is poorly understood. Activated platelets can release pro-fibrotic mediators that have the potential to contribute to lung fibrosis. We determine platelet reactivity in subjects with IPF compared to age-matched controls.Whole blood flow cytometry was used to measure platelet-monocyte aggregate formation, platelet P-selectin expression and platelet fibrinogen binding at basal levels and following stimulation with platelet agonists. A plasma swap approach was used to assess the effect of IPF plasma on control platelets.Subjects with IPF showed greater platelet reactivity than controls. Platelet P-selectin expression was significantly greater in IPF patients than controls following stimulation with 0.1 µM ADP (1.9% positive ±0.5 (mean ± SEM) versus 0.7%±0.1; p = 0.03), 1 µM ADP (9.8%±1.3 versus 3.3%±0.8; p<0.01) and 10 µM ADP (41.3%±4.2 versus 22.5%±2.6; p<0.01). Platelet fibrinogen binding was also increased, and platelet activation resulted in increased platelet-monocyte aggregate formation in IPF patients. Re-suspension of control platelets in plasma taken from subjects with IPF resulted in increased platelet activation compared to control plasma.IPF patients exhibit increased platelet reactivity compared with controls. This hyperactivity may result from the plasma environment since control platelets exhibit increased activation when exposed to IPF plasma.http://europepmc.org/articles/PMC4206466?pdf=render |
| spellingShingle | Michael G Crooks Ahmed Fahim Khalid M Naseem Alyn H Morice Simon P Hart Increased platelet reactivity in idiopathic pulmonary fibrosis is mediated by a plasma factor. PLoS ONE |
| title | Increased platelet reactivity in idiopathic pulmonary fibrosis is mediated by a plasma factor. |
| title_full | Increased platelet reactivity in idiopathic pulmonary fibrosis is mediated by a plasma factor. |
| title_fullStr | Increased platelet reactivity in idiopathic pulmonary fibrosis is mediated by a plasma factor. |
| title_full_unstemmed | Increased platelet reactivity in idiopathic pulmonary fibrosis is mediated by a plasma factor. |
| title_short | Increased platelet reactivity in idiopathic pulmonary fibrosis is mediated by a plasma factor. |
| title_sort | increased platelet reactivity in idiopathic pulmonary fibrosis is mediated by a plasma factor |
| url | http://europepmc.org/articles/PMC4206466?pdf=render |
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