Homologous recombination-mediated cloning and manipulation of genomic DNA regions using Gateway and recombineering systems

<p>Abstract</p> <p>Background</p> <p>Employing genomic DNA clones to characterise gene attributes has several advantages over the use of cDNA clones, including the presence of native transcription and translation regulatory sequences as well as a representation of the c...

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Bibliographic Details
Main Authors: Kagale Sateesh, Yang Wen, Rozwadowski Kevin
Format: Article
Language:English
Published: BMC 2008-11-01
Series:BMC Biotechnology
Online Access:http://www.biomedcentral.com/1472-6750/8/88