Nerve ECM and PLA-PCL based electrospun bilayer nerve conduit for nerve regeneration
Introduction: The porcine nerve-derived extracellular matrix (ECM) fabricated as films has good performance in peripheral nerve regeneration. However, when constructed as conduits to bridge nerve defects, ECM lacks sufficient mechanical strength.Methods: In this study, a novel electrospun bilayer-st...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1103435/full |
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| author | Xiaoyan Mao Ting Li Ting Li Junqiu Cheng Meihan Tao Zhiyuan Li Yizhan Ma Rabia Javed Jie Bao Fang Liang Weihong Guo Xiaohong Tian Jun Fan Tianhao Yu Qiang Ao Qiang Ao Qiang Ao |
| author_facet | Xiaoyan Mao Ting Li Ting Li Junqiu Cheng Meihan Tao Zhiyuan Li Yizhan Ma Rabia Javed Jie Bao Fang Liang Weihong Guo Xiaohong Tian Jun Fan Tianhao Yu Qiang Ao Qiang Ao Qiang Ao |
| author_sort | Xiaoyan Mao |
| collection | DOAJ |
| description | Introduction: The porcine nerve-derived extracellular matrix (ECM) fabricated as films has good performance in peripheral nerve regeneration. However, when constructed as conduits to bridge nerve defects, ECM lacks sufficient mechanical strength.Methods: In this study, a novel electrospun bilayer-structured nerve conduit (BNC) with outer poly (L-lactic acid-co-ε-caprolactone) (PLA-PCL) and inner ECM was fabricated for nerve regeneration. The composition, structure, and mechanical strength of BNC were characterized. Then BNC biosafety was evaluated by cytotoxicity, subcutaneous implantation, and cell affinity tests. Furthermore, BNC was used to bridge 10-mm rat sciatic nerve defect, and nerve functional recovery was assessed by walking track, electrophysiology, and histomorphology analyses.Results: Our results demonstrate that BNC has a network of nanofibers and retains some bioactive molecules, including collagen I, collagen IV, laminin, fibronectin, glycosaminoglycans, nerve growth factor, and brain-derived neurotrophic factor. Biomechanical analysis proves that PLA-PCL improves the BNC mechanical properties, compared with single ECM conduit (ENC). The functional evaluation of in vivo results indicated that BNC is more effective in nerve regeneration than PLA-PCL conduit or ENC.Discussion: In conclusion, BNC not only retains the good biocompatibility and bioactivity of ECM, but also obtains the appropriate mechanical strength from PLA-PCL, which has great potential for clinical repair of nerve defects. |
| first_indexed | 2024-04-10T06:17:44Z |
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| id | doaj.art-c8cee3e0a0884ccba24ab938d946cbcb |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-10T06:17:44Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-c8cee3e0a0884ccba24ab938d946cbcb2023-03-02T06:06:19ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-03-011110.3389/fbioe.2023.11034351103435Nerve ECM and PLA-PCL based electrospun bilayer nerve conduit for nerve regenerationXiaoyan Mao0Ting Li1Ting Li2Junqiu Cheng3Meihan Tao4Zhiyuan Li5Yizhan Ma6Rabia Javed7Jie Bao8Fang Liang9Weihong Guo10Xiaohong Tian11Jun Fan12Tianhao Yu13Qiang Ao14Qiang Ao15Qiang Ao16Department of Tissue Engineering, China Medical University, Shenyang, ChinaDepartment of Tissue Engineering, China Medical University, Shenyang, ChinaDepartment of Laboratory Medicine, Shengjing Hospital of China Medical University, Shenyang, ChinaNational Engineering Research Center for Biomaterials, Sichuan University, Chengdu, ChinaDepartment of Tissue Engineering, China Medical University, Shenyang, ChinaDepartment of Tissue Engineering, China Medical University, Shenyang, ChinaDepartment of Tissue Engineering, China Medical University, Shenyang, ChinaDepartment of Tissue Engineering, China Medical University, Shenyang, ChinaDepartment of Tissue Engineering, China Medical University, Shenyang, ChinaDepartment of Tissue Engineering, China Medical University, Shenyang, ChinaFuwai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaDepartment of Tissue Engineering, China Medical University, Shenyang, ChinaDepartment of Tissue Engineering, China Medical University, Shenyang, ChinaLiaoning Provincial Key Laboratory of Oral Diseases, The VIP Department, School and Hospital of Stomatology, China Medical University, Shenyang, ChinaDepartment of Tissue Engineering, China Medical University, Shenyang, ChinaNational Engineering Research Center for Biomaterials, Sichuan University, Chengdu, ChinaInstitute of Regulatory Science for Medical Device, Sichuan University, Chengdu, ChinaIntroduction: The porcine nerve-derived extracellular matrix (ECM) fabricated as films has good performance in peripheral nerve regeneration. However, when constructed as conduits to bridge nerve defects, ECM lacks sufficient mechanical strength.Methods: In this study, a novel electrospun bilayer-structured nerve conduit (BNC) with outer poly (L-lactic acid-co-ε-caprolactone) (PLA-PCL) and inner ECM was fabricated for nerve regeneration. The composition, structure, and mechanical strength of BNC were characterized. Then BNC biosafety was evaluated by cytotoxicity, subcutaneous implantation, and cell affinity tests. Furthermore, BNC was used to bridge 10-mm rat sciatic nerve defect, and nerve functional recovery was assessed by walking track, electrophysiology, and histomorphology analyses.Results: Our results demonstrate that BNC has a network of nanofibers and retains some bioactive molecules, including collagen I, collagen IV, laminin, fibronectin, glycosaminoglycans, nerve growth factor, and brain-derived neurotrophic factor. Biomechanical analysis proves that PLA-PCL improves the BNC mechanical properties, compared with single ECM conduit (ENC). The functional evaluation of in vivo results indicated that BNC is more effective in nerve regeneration than PLA-PCL conduit or ENC.Discussion: In conclusion, BNC not only retains the good biocompatibility and bioactivity of ECM, but also obtains the appropriate mechanical strength from PLA-PCL, which has great potential for clinical repair of nerve defects.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1103435/fullperipheral nerve regenerationelectrospunECMbilayer structurenerve conduit |
| spellingShingle | Xiaoyan Mao Ting Li Ting Li Junqiu Cheng Meihan Tao Zhiyuan Li Yizhan Ma Rabia Javed Jie Bao Fang Liang Weihong Guo Xiaohong Tian Jun Fan Tianhao Yu Qiang Ao Qiang Ao Qiang Ao Nerve ECM and PLA-PCL based electrospun bilayer nerve conduit for nerve regeneration Frontiers in Bioengineering and Biotechnology peripheral nerve regeneration electrospun ECM bilayer structure nerve conduit |
| title | Nerve ECM and PLA-PCL based electrospun bilayer nerve conduit for nerve regeneration |
| title_full | Nerve ECM and PLA-PCL based electrospun bilayer nerve conduit for nerve regeneration |
| title_fullStr | Nerve ECM and PLA-PCL based electrospun bilayer nerve conduit for nerve regeneration |
| title_full_unstemmed | Nerve ECM and PLA-PCL based electrospun bilayer nerve conduit for nerve regeneration |
| title_short | Nerve ECM and PLA-PCL based electrospun bilayer nerve conduit for nerve regeneration |
| title_sort | nerve ecm and pla pcl based electrospun bilayer nerve conduit for nerve regeneration |
| topic | peripheral nerve regeneration electrospun ECM bilayer structure nerve conduit |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1103435/full |
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