Ultrasensitive and visual detection of Feline herpesvirus type-1 and Feline calicivirus using one-tube dRPA-Cas12a/Cas13a assay

Ultrasensitive and visual detection of Feline herpesvirus type-1 and Feline calicivirus using one-tube dRPA-Cas12a/Cas13a assay

Abstract Background Feline herpesvirus type 1 (FHV) and Feline calicivirus (FCV) are the primary co-infecting pathogens that cause upper respiratory tract disease in cats. However, there are currently no visual detection assays available for on-site testing. Here, we develop an ultrasensitive and vi...

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Bibliographic Details
Main Authors: Fumei Jiang, Yunjia Liu, Xiaonong Yang, Yan Li, Jian Huang
Format: Article
Language:English
Published: BMC 2024-03-01
Series:BMC Veterinary Research
Subjects:
FHV-1
FCV
Simultaneous detection
RPA
Hybrid CRISPR/Cas effectors
Visual modality
Online Access:https://doi.org/10.1186/s12917-024-03953-9

Internet

https://doi.org/10.1186/s12917-024-03953-9

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