Assessment of Mosaicism and Detection of Cryptic Alleles in CRISPR/Cas9-Engineered Neurofibromatosis Type 1 and TP53 Mutant Porcine Models Reveals Overlooked Challenges in Precision Modeling of Human Diseases

Assessment of Mosaicism and Detection of Cryptic Alleles in CRISPR/Cas9-Engineered Neurofibromatosis Type 1 and TP53 Mutant Porcine Models Reveals Overlooked Challenges in Precision Modeling of Human Diseases

Genome editing in pigs has been made efficient, practical, and economically viable by the CRISPR/Cas9 platform, representing a promising new era in translational modeling of human disease for research and preclinical development of therapies and devices. Porcine embryo microinjection provides a univ...

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Bibliographic Details
Main Authors: Clifford Dustin Rubinstein, Dalton T. McLean, Brent P. Lehman, Jennifer J. Meudt, Dominic T. Schomberg, Kathy J. Krentz, Jamie L. Reichert, Mark B. Meyer, Marie Adams, Charles M. Konsitzke, Dhanansayan Shanmuganayagam
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-09-01
Series:Frontiers in Genetics
Subjects:
neurofibromatosis type 1
CRISPR
swine
genetic engineering
microinjection
cryptic allele
Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2021.721045/full

Internet

https://www.frontiersin.org/articles/10.3389/fgene.2021.721045/full

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