Optimization of composite cryoprotectant for freeze-drying Bifidobacterium bifidum BB01 by response surface methodology
Freeze drying has been well applied in the preparation of high-efficiency probiotic powders. However, the process is generally accompanied by probiotic viability deficiency, which is the bottleneck for further application. To improve the viability of Bifidobacterium bifidum BB01 during freeze-drying...
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| Format: | Article |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1603157 |
| _version_ | 1818008668750217216 |
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| author | He Chen Mengqi Tian Li Chen Xiuxiu Cui Jiangpeng Meng Guowei Shu |
| author_facet | He Chen Mengqi Tian Li Chen Xiuxiu Cui Jiangpeng Meng Guowei Shu |
| author_sort | He Chen |
| collection | DOAJ |
| description | Freeze drying has been well applied in the preparation of high-efficiency probiotic powders. However, the process is generally accompanied by probiotic viability deficiency, which is the bottleneck for further application. To improve the viability of Bifidobacterium bifidum BB01 during freeze-drying, we optimized the cryoprotectant of B. bifidum BB01 by response surface methodology (RSM) with a Central Composite Design (CCD). In this study, two values of B. bifidum BB01 with different protectant factors were investigated, including freeze-drying survival rate and the viable counts of per unit weight of freeze-dried powder. The optimized cryoprotectants were obtained as follows: glycine of 5.5%, sodium bicarbonate of 0.8%, xylo-oligosaccharides of 7%, arginine of 4.5% and skim milk of 25%. The survival rate and the viable counts of per unit weight of powder were 90.37 ± 1.9% and (2.78 ± 0.13) × 1011cfu·g−1, respectively, both close to the predicted value (88.58% and 2.71 × 1011 cfu·g−1). Our research demonstrated that RSM was successful in optimizing composite cryoprotectant for freeze-dried powder of B. bifidum which can as well protect the probiotic cells. |
| first_indexed | 2024-04-14T05:32:12Z |
| format | Article |
| id | doaj.art-c914580165a640ceb9af4e6b9eb536ea |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-04-14T05:32:12Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-c914580165a640ceb9af4e6b9eb536ea2022-12-22T02:09:45ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014711559156910.1080/21691401.2019.1603157Optimization of composite cryoprotectant for freeze-drying Bifidobacterium bifidum BB01 by response surface methodologyHe Chen0Mengqi Tian1Li Chen2Xiuxiu Cui3Jiangpeng Meng4Guowei Shu5School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an, PR ChinaSchool of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an, PR ChinaCollege of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an, PR ChinaDepartment of Research and Development, Xi’an Baiyue Goat Milk Corp., Ltd, Xi’an, PR ChinaDepartment of Research and Development, Xi’an Baiyue Goat Milk Corp., Ltd, Xi’an, PR ChinaSchool of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an, PR ChinaFreeze drying has been well applied in the preparation of high-efficiency probiotic powders. However, the process is generally accompanied by probiotic viability deficiency, which is the bottleneck for further application. To improve the viability of Bifidobacterium bifidum BB01 during freeze-drying, we optimized the cryoprotectant of B. bifidum BB01 by response surface methodology (RSM) with a Central Composite Design (CCD). In this study, two values of B. bifidum BB01 with different protectant factors were investigated, including freeze-drying survival rate and the viable counts of per unit weight of freeze-dried powder. The optimized cryoprotectants were obtained as follows: glycine of 5.5%, sodium bicarbonate of 0.8%, xylo-oligosaccharides of 7%, arginine of 4.5% and skim milk of 25%. The survival rate and the viable counts of per unit weight of powder were 90.37 ± 1.9% and (2.78 ± 0.13) × 1011cfu·g−1, respectively, both close to the predicted value (88.58% and 2.71 × 1011 cfu·g−1). Our research demonstrated that RSM was successful in optimizing composite cryoprotectant for freeze-dried powder of B. bifidum which can as well protect the probiotic cells.https://www.tandfonline.com/doi/10.1080/21691401.2019.1603157Composite cryoprotectantBifidobacterium bifidumfreeze dryingresponse surface methodology |
| spellingShingle | He Chen Mengqi Tian Li Chen Xiuxiu Cui Jiangpeng Meng Guowei Shu Optimization of composite cryoprotectant for freeze-drying Bifidobacterium bifidum BB01 by response surface methodology Artificial Cells, Nanomedicine, and Biotechnology Composite cryoprotectant Bifidobacterium bifidum freeze drying response surface methodology |
| title | Optimization of composite cryoprotectant for freeze-drying Bifidobacterium bifidum BB01 by response surface methodology |
| title_full | Optimization of composite cryoprotectant for freeze-drying Bifidobacterium bifidum BB01 by response surface methodology |
| title_fullStr | Optimization of composite cryoprotectant for freeze-drying Bifidobacterium bifidum BB01 by response surface methodology |
| title_full_unstemmed | Optimization of composite cryoprotectant for freeze-drying Bifidobacterium bifidum BB01 by response surface methodology |
| title_short | Optimization of composite cryoprotectant for freeze-drying Bifidobacterium bifidum BB01 by response surface methodology |
| title_sort | optimization of composite cryoprotectant for freeze drying bifidobacterium bifidum bb01 by response surface methodology |
| topic | Composite cryoprotectant Bifidobacterium bifidum freeze drying response surface methodology |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1603157 |
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