Cloning Full-Length, Cap-Trapper-Selected cDNAs by Using the Single-Strand Linker Ligation Method
We have developed the single-strand linker ligation method (SSLLM), which uses DNA ligase to add a dsDNA linker to singlestranded (ss) full-length cDNA. The linkers have random 6-bp (dN6 or dGN5) 3′ overhangs that can ligate to any cDNA sequence, thereby facilitating the production of cDNA libraries...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2001-06-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/01306st01 |