Armillariella tabescens methanol extract ameliorates ulcerative colitis via inhibiting TLR4/NF-κB and NLRP3 activation and mediating intestinal barrier integrity
Armillariella tabescens is a traditional Chinese medicinal and edible fungus which holds importance for treatment of gastrointestinal disorders. This study was aimed to evaluate the effect of methanol extract of A. tabescens (AME) in dextran sulfate sodium (DSS) induced ulcerative colitis (UC). The...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622003711 |
| _version_ | 1811201814946643968 |
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| author | Wen-Na Zhang Yuan-Yuan Li Jia-Jia Xu Lu-Lu Shi Lei Chen Yong-Ming Lu Qing-Xi Wu Jian-Guang Luo Yan Chen |
| author_facet | Wen-Na Zhang Yuan-Yuan Li Jia-Jia Xu Lu-Lu Shi Lei Chen Yong-Ming Lu Qing-Xi Wu Jian-Guang Luo Yan Chen |
| author_sort | Wen-Na Zhang |
| collection | DOAJ |
| description | Armillariella tabescens is a traditional Chinese medicinal and edible fungus which holds importance for treatment of gastrointestinal disorders. This study was aimed to evaluate the effect of methanol extract of A. tabescens (AME) in dextran sulfate sodium (DSS) induced ulcerative colitis (UC). The results showed that AME significantly attenuated UC symptoms, suppressed the activity of myeloperoxidase (MPO), decreased the levels of LPS, TNF-α, IL-1β, IL-18, and increased the level of IL-10 in serum. Besides, AME could improve the expression of tight junction proteins ZO-1, occludin and claudin-1. Moreover, AME inhibited the activation of TLR4/NF-κB and NLRP3 signaling pathways in colon tissue. In addition, eight compounds were identification in AME by high performance liquid chromatography (HPLC)-high resolution mass spectroscopy (HRMS). Therefore, this study indicated that AME was potential to be further developed into a suitable functional food additive or promising therapeutic agent for UC treatment. |
| first_indexed | 2024-04-12T02:28:16Z |
| format | Article |
| id | doaj.art-c94c5d538cfb4685b7c9e4269172c9d4 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-12T02:28:16Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-c94c5d538cfb4685b7c9e4269172c9d42022-12-22T03:51:54ZengElsevierJournal of Functional Foods1756-46462022-12-0199105301Armillariella tabescens methanol extract ameliorates ulcerative colitis via inhibiting TLR4/NF-κB and NLRP3 activation and mediating intestinal barrier integrityWen-Na Zhang0Yuan-Yuan Li1Jia-Jia Xu2Lu-Lu Shi3Lei Chen4Yong-Ming Lu5Qing-Xi Wu6Jian-Guang Luo7Yan Chen8School of Life Sciences, Anhui University, Hefei 230601, Anhui, China; Key Laboratory of Eco‐engineering and Biotechnology of Anhui Province, Anhui University, Hefei 230601, ChinaSchool of Life Sciences, Anhui University, Hefei 230601, Anhui, ChinaSchool of Life Sciences, Anhui University, Hefei 230601, Anhui, ChinaSchool of Life Sciences, Anhui University, Hefei 230601, Anhui, ChinaSchool of Life Sciences, Anhui University, Hefei 230601, Anhui, ChinaSchool of Life Sciences, Anhui University, Hefei 230601, Anhui, China; Key Laboratory of Eco‐engineering and Biotechnology of Anhui Province, Anhui University, Hefei 230601, ChinaSchool of Life Sciences, Anhui University, Hefei 230601, Anhui, China; Key Laboratory of Eco‐engineering and Biotechnology of Anhui Province, Anhui University, Hefei 230601, ChinaState Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, Jiangsu, China; Corresponding authors at: School of Life Sciences, Anhui University, Hefei 230601, Anhui, China (Y. Chen).School of Life Sciences, Anhui University, Hefei 230601, Anhui, China; Key Laboratory of Eco‐engineering and Biotechnology of Anhui Province, Anhui University, Hefei 230601, China; Corresponding authors at: School of Life Sciences, Anhui University, Hefei 230601, Anhui, China (Y. Chen).Armillariella tabescens is a traditional Chinese medicinal and edible fungus which holds importance for treatment of gastrointestinal disorders. This study was aimed to evaluate the effect of methanol extract of A. tabescens (AME) in dextran sulfate sodium (DSS) induced ulcerative colitis (UC). The results showed that AME significantly attenuated UC symptoms, suppressed the activity of myeloperoxidase (MPO), decreased the levels of LPS, TNF-α, IL-1β, IL-18, and increased the level of IL-10 in serum. Besides, AME could improve the expression of tight junction proteins ZO-1, occludin and claudin-1. Moreover, AME inhibited the activation of TLR4/NF-κB and NLRP3 signaling pathways in colon tissue. In addition, eight compounds were identification in AME by high performance liquid chromatography (HPLC)-high resolution mass spectroscopy (HRMS). Therefore, this study indicated that AME was potential to be further developed into a suitable functional food additive or promising therapeutic agent for UC treatment.http://www.sciencedirect.com/science/article/pii/S1756464622003711Armillariella tabescensUlcerative colitisTight junction proteinsTLR4/NF-κBNLRP3 |
| spellingShingle | Wen-Na Zhang Yuan-Yuan Li Jia-Jia Xu Lu-Lu Shi Lei Chen Yong-Ming Lu Qing-Xi Wu Jian-Guang Luo Yan Chen Armillariella tabescens methanol extract ameliorates ulcerative colitis via inhibiting TLR4/NF-κB and NLRP3 activation and mediating intestinal barrier integrity Journal of Functional Foods Armillariella tabescens Ulcerative colitis Tight junction proteins TLR4/NF-κB NLRP3 |
| title | Armillariella tabescens methanol extract ameliorates ulcerative colitis via inhibiting TLR4/NF-κB and NLRP3 activation and mediating intestinal barrier integrity |
| title_full | Armillariella tabescens methanol extract ameliorates ulcerative colitis via inhibiting TLR4/NF-κB and NLRP3 activation and mediating intestinal barrier integrity |
| title_fullStr | Armillariella tabescens methanol extract ameliorates ulcerative colitis via inhibiting TLR4/NF-κB and NLRP3 activation and mediating intestinal barrier integrity |
| title_full_unstemmed | Armillariella tabescens methanol extract ameliorates ulcerative colitis via inhibiting TLR4/NF-κB and NLRP3 activation and mediating intestinal barrier integrity |
| title_short | Armillariella tabescens methanol extract ameliorates ulcerative colitis via inhibiting TLR4/NF-κB and NLRP3 activation and mediating intestinal barrier integrity |
| title_sort | armillariella tabescens methanol extract ameliorates ulcerative colitis via inhibiting tlr4 nf κb and nlrp3 activation and mediating intestinal barrier integrity |
| topic | Armillariella tabescens Ulcerative colitis Tight junction proteins TLR4/NF-κB NLRP3 |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622003711 |
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