An improvised one-step OptiPrep cushion ultracentrifugation method for outer membrane vesicles isolation of Klebsiella pneumoniae

An improvised one-step OptiPrep cushion ultracentrifugation method for outer membrane vesicles isolation of Klebsiella pneumoniae

Abstract Background Extracellular vesicles (EVs) play a crucial role in intraspecies and interspecies communication, significantly influencing physiological and pathological processes. Outer membrane vesicles (OMVs) secreted by Gram-negative bacteria are rich in components from the parent cells and...

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Bibliographic Details
Main Authors: Ping Li, Ming Lu, Tingxiu Peng, Yifan Wu, Lanlan Zhu, Yang Liu, Wei Zhang, Tianxin Xiang
Format: Article
Language:English
Published: BMC 2024-12-01
Series:BMC Microbiology
Subjects:
Klebsiella pneumoniae
Outer membrane vesicles (OMVs)
Centrifugation techniques
One-step OptiPrep density gradient centrifugation
Differential centrifugation
Online Access:https://doi.org/10.1186/s12866-024-03649-y

Internet

https://doi.org/10.1186/s12866-024-03649-y

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