Morinda officinalis oligosaccharides mitigate chronic mild stress-induced inflammation and depression-like behaviour by deactivating the MyD88/PI3K pathway via E2F2
Morinda officinalis oligosaccharides (MOs) are natural herbal extracts that have been shown to exert antidepressant effects. However, the mechanism of this effect remains unclear. Here, we explored the mechanism by which MOs improved experimental depression. Using a chronic mild stress (CMS) murine...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.855964/full |
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| author | Zhen-Hua Zhu Xu-Yuan Yin Tu-Sun Xu Wei-Wei Tao Guang-Da Yao Pei-Jie Wang Qi Qi Qiu-Fang Jia Jing Wang Yue Zhu Li Hui |
| author_facet | Zhen-Hua Zhu Xu-Yuan Yin Tu-Sun Xu Wei-Wei Tao Guang-Da Yao Pei-Jie Wang Qi Qi Qiu-Fang Jia Jing Wang Yue Zhu Li Hui |
| author_sort | Zhen-Hua Zhu |
| collection | DOAJ |
| description | Morinda officinalis oligosaccharides (MOs) are natural herbal extracts that have been shown to exert antidepressant effects. However, the mechanism of this effect remains unclear. Here, we explored the mechanism by which MOs improved experimental depression. Using a chronic mild stress (CMS) murine model, we examined whether MOs could protect against depressive-like behaviour. Lipopolysaccharide (LPS)- and ATP-treated BV2 cells were used to examine the potential mechanism by which MOs mediate the inflammatory response. We found that MOs prevented the CMS-induced reduction in the sucrose preference ratio in the sucrose preference test (SPT) and shortened the immobility durations in both the tail suspension test (TST) and forced swim test (FST). We also noticed that MOs suppressed inflammatory effects by deactivating the MyD88/PI3K pathway via E2F2 in CMS mice or LPS- and ATP-stimulated BV2 cells. Furthermore, overexpression of E2F2 blunted the beneficial effects of MOs in vitro. Collectively, these data showed that MOs exerted antidepressant effects in CMS mice by targeting E2F2-mediated MyD88/PI3K signalling pathway. |
| first_indexed | 2024-04-13T13:17:44Z |
| format | Article |
| id | doaj.art-c98557a5022b4592b64e4dc42c1edf7f |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-13T13:17:44Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-c98557a5022b4592b64e4dc42c1edf7f2022-12-22T02:45:24ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-08-011310.3389/fphar.2022.855964855964Morinda officinalis oligosaccharides mitigate chronic mild stress-induced inflammation and depression-like behaviour by deactivating the MyD88/PI3K pathway via E2F2Zhen-Hua Zhu0Xu-Yuan Yin1Tu-Sun Xu2Wei-Wei Tao3Guang-Da Yao4Pei-Jie Wang5Qi Qi6Qiu-Fang Jia7Jing Wang8Yue Zhu9Li Hui10Research Center of Biological Psychiatry, Suzhou Guangji Hospital, Medical College of Soochow University, Suzhou, ChinaResearch Center of Biological Psychiatry, Suzhou Guangji Hospital, Medical College of Soochow University, Suzhou, ChinaResearch Center of Biological Psychiatry, Suzhou Guangji Hospital, Medical College of Soochow University, Suzhou, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing, ChinaNingxia Medical University, Ningxia Key Laboratory of Cerebrocranial Disease, Incubation Base of National Key Laboratory, Nanjing, ChinaResearch Center of Biological Psychiatry, Suzhou Guangji Hospital, Medical College of Soochow University, Suzhou, ChinaResearch Center of Biological Psychiatry, Suzhou Guangji Hospital, Medical College of Soochow University, Suzhou, ChinaResearch Center of Biological Psychiatry, Suzhou Guangji Hospital, Medical College of Soochow University, Suzhou, ChinaResearch Center of Biological Psychiatry, Suzhou Guangji Hospital, Medical College of Soochow University, Suzhou, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing, ChinaResearch Center of Biological Psychiatry, Suzhou Guangji Hospital, Medical College of Soochow University, Suzhou, ChinaMorinda officinalis oligosaccharides (MOs) are natural herbal extracts that have been shown to exert antidepressant effects. However, the mechanism of this effect remains unclear. Here, we explored the mechanism by which MOs improved experimental depression. Using a chronic mild stress (CMS) murine model, we examined whether MOs could protect against depressive-like behaviour. Lipopolysaccharide (LPS)- and ATP-treated BV2 cells were used to examine the potential mechanism by which MOs mediate the inflammatory response. We found that MOs prevented the CMS-induced reduction in the sucrose preference ratio in the sucrose preference test (SPT) and shortened the immobility durations in both the tail suspension test (TST) and forced swim test (FST). We also noticed that MOs suppressed inflammatory effects by deactivating the MyD88/PI3K pathway via E2F2 in CMS mice or LPS- and ATP-stimulated BV2 cells. Furthermore, overexpression of E2F2 blunted the beneficial effects of MOs in vitro. Collectively, these data showed that MOs exerted antidepressant effects in CMS mice by targeting E2F2-mediated MyD88/PI3K signalling pathway.https://www.frontiersin.org/articles/10.3389/fphar.2022.855964/fulldepressioninflammationMyD88/PI3KE2F2morinda officinalis oligosaccharides |
| spellingShingle | Zhen-Hua Zhu Xu-Yuan Yin Tu-Sun Xu Wei-Wei Tao Guang-Da Yao Pei-Jie Wang Qi Qi Qiu-Fang Jia Jing Wang Yue Zhu Li Hui Morinda officinalis oligosaccharides mitigate chronic mild stress-induced inflammation and depression-like behaviour by deactivating the MyD88/PI3K pathway via E2F2 Frontiers in Pharmacology depression inflammation MyD88/PI3K E2F2 morinda officinalis oligosaccharides |
| title | Morinda officinalis oligosaccharides mitigate chronic mild stress-induced inflammation and depression-like behaviour by deactivating the MyD88/PI3K pathway via E2F2 |
| title_full | Morinda officinalis oligosaccharides mitigate chronic mild stress-induced inflammation and depression-like behaviour by deactivating the MyD88/PI3K pathway via E2F2 |
| title_fullStr | Morinda officinalis oligosaccharides mitigate chronic mild stress-induced inflammation and depression-like behaviour by deactivating the MyD88/PI3K pathway via E2F2 |
| title_full_unstemmed | Morinda officinalis oligosaccharides mitigate chronic mild stress-induced inflammation and depression-like behaviour by deactivating the MyD88/PI3K pathway via E2F2 |
| title_short | Morinda officinalis oligosaccharides mitigate chronic mild stress-induced inflammation and depression-like behaviour by deactivating the MyD88/PI3K pathway via E2F2 |
| title_sort | morinda officinalis oligosaccharides mitigate chronic mild stress induced inflammation and depression like behaviour by deactivating the myd88 pi3k pathway via e2f2 |
| topic | depression inflammation MyD88/PI3K E2F2 morinda officinalis oligosaccharides |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.855964/full |
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