Simultaneous and ultrafast detection of pan-SARS-coronaviruses and influenza A/B viruses by a novel multiplex real-time RT-PCR assay

Simultaneous and ultrafast detection of pan-SARS-coronaviruses and influenza A/B viruses by a novel multiplex real-time RT-PCR assay

Here we report an ultrafast quadruplex RT-qPCR assay with robust diagnostic ability to detect and distinguish pan-SARS-CoVs and influenza A/B viruses within 35 min. This quadruplex RT-qPCR assay comprised of one novel RNA-based internal control targeting human β2-microglobulin (B2M) for process accu...

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Bibliographic Details
Main Authors: Changping Xu, Zhengyang Wang, Beibei Yu, Zhenhuang Pan, Jun Ni, Yan Feng, Shiwang Huang, Maomao Wu, Jiancang Zhou, Lei Fang, Zhiwei Wu
Format: Article
Language:English
Published: Elsevier 2024-08-01
Series:Virus Research
Subjects:
Molecular diagnostics
Co-surveillance
SARS-CoV-2
Viral spillover
SARS
Influenza
Online Access:http://www.sciencedirect.com/science/article/pii/S0168170224001035

Internet

http://www.sciencedirect.com/science/article/pii/S0168170224001035

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