Generation of Red-Shifted Cameleons for Imaging Ca2+ Dynamics of the Endoplasmic Reticulum

Generation of Red-Shifted Cameleons for Imaging Ca2+ Dynamics of the Endoplasmic Reticulum

Cameleons are sophisticated genetically encoded fluorescent probes that allow quantifying cellular Ca2+ signals. The probes are based on Förster resonance energy transfer (FRET) between terminally located fluorescent proteins (FPs), which move together upon binding of Ca2+ to the central calmodulin...

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Bibliographic Details
Main Authors: Markus Waldeck-Weiermair, Helmut Bischof, Sandra Blass, Andras T. Deak, Christiane Klec, Thomas Graier, Clara Roller, Rene Rost, Emrah Eroglu, Benjamin Gottschalk, Nicole A. Hofmann, Wolfgang F. Graier, Roland Malli
Format: Article
Language:English
Published: MDPI AG 2015-06-01
Series:Sensors
Subjects:
Förster resonance energy transfer
FRET
calcium
endoplasmic reticulum
store operated calcium entry
SOCE
clover
mRuby2
dissociation constant
Online Access:http://www.mdpi.com/1424-8220/15/6/13052

Internet

http://www.mdpi.com/1424-8220/15/6/13052

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