Effects of the PARP inhibitor Niraparib on the radiosensitivity of human lung and cervical cancer cells
Objective: To investigate the effect of Niraparib, a clinically approved PARP inhibitor on the radiosensitivity of human lung and cervical cancer cells, and preliminarily explore the underlying mechanism. Methods: The human lung cancer cell line A549 and human cervical cancer cell line Siha were bot...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Radiation Medicine and Protection |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666555723000229 |
| _version_ | 1797798629101010944 |
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| author | Yue Feng Shiyi Pi Yuwei Li Yu Zhang Hongwei Zeng E. Xiangyu Yang Zhang Jingping Yu |
| author_facet | Yue Feng Shiyi Pi Yuwei Li Yu Zhang Hongwei Zeng E. Xiangyu Yang Zhang Jingping Yu |
| author_sort | Yue Feng |
| collection | DOAJ |
| description | Objective: To investigate the effect of Niraparib, a clinically approved PARP inhibitor on the radiosensitivity of human lung and cervical cancer cells, and preliminarily explore the underlying mechanism. Methods: The human lung cancer cell line A549 and human cervical cancer cell line Siha were both treated with Niraparib for 1 h, or X-ray irradiation with 4 Gy, or Niraparib for 1 h combined with X-ray irradiation with 4 Gy. The effect was examined via measurements of cell proliferation by the cell counting kit-8 assay, and cell viability was detected by clone formation assays. Cell apoptosis and cell cycle distribution were investigated by flow cytometry. Results: In human lung and cervical cancer cell lines, Niraparib combined with radiation therapy significantly inhibited cell proliferation. The proportion of apoptotic cells in cell lines treated with Niraparib plus radiation (the combination group) was significantly higher compared with control, radiation, and Niraparib groups (P < 0.05). Additionally, the proportion of A549 cells in the G2/M phase was significantly increased in the combination group compared with the radiation group (P < 0.05). Conclusion: PARP inhibitor Niraparib increases the radiosensitivity of tumorcells, promotes their apoptosis, and induces cell cycle redistribution. The possible mechanism is associated with the inhibition of radiation-induced DNA damage repair. |
| first_indexed | 2024-03-13T04:06:42Z |
| format | Article |
| id | doaj.art-c9c95249d8624035aaec3c5fa620af9c |
| institution | Directory Open Access Journal |
| issn | 2666-5557 |
| language | English |
| last_indexed | 2024-03-13T04:06:42Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Radiation Medicine and Protection |
| spelling | doaj.art-c9c95249d8624035aaec3c5fa620af9c2023-06-21T07:00:39ZengElsevierRadiation Medicine and Protection2666-55572023-06-01429397Effects of the PARP inhibitor Niraparib on the radiosensitivity of human lung and cervical cancer cellsYue Feng0Shiyi Pi1Yuwei Li2Yu Zhang3Hongwei Zeng4E. Xiangyu5Yang Zhang6Jingping Yu7Department of Radiotherapy, Shuguang Hospital Affiliated to Shanghai University of Chinese Traditional Medicine, Shanghai 201203, ChinaDepartment of Radiotherapy, Chengdu Women and Children's Central Hospital, Chengdu 610000, ChinaDepartment of Radiotherapy, Puyang People's Hospital, Puyang Henan, 457000, ChinaDepartment of Radiotherapy, Shuguang Hospital Affiliated to Shanghai University of Chinese Traditional Medicine, Shanghai 201203, ChinaDepartment of Radiotherapy, Shuguang Hospital Affiliated to Shanghai University of Chinese Traditional Medicine, Shanghai 201203, ChinaDepartment of Radiotherapy, Shuguang Hospital Affiliated to Shanghai University of Chinese Traditional Medicine, Shanghai 201203, ChinaDepartment of Radiotherapy, Shuguang Hospital Affiliated to Shanghai University of Chinese Traditional Medicine, Shanghai 201203, ChinaDepartment of Radiotherapy, Shuguang Hospital Affiliated to Shanghai University of Chinese Traditional Medicine, Shanghai 201203, China; Corresponding author.Objective: To investigate the effect of Niraparib, a clinically approved PARP inhibitor on the radiosensitivity of human lung and cervical cancer cells, and preliminarily explore the underlying mechanism. Methods: The human lung cancer cell line A549 and human cervical cancer cell line Siha were both treated with Niraparib for 1 h, or X-ray irradiation with 4 Gy, or Niraparib for 1 h combined with X-ray irradiation with 4 Gy. The effect was examined via measurements of cell proliferation by the cell counting kit-8 assay, and cell viability was detected by clone formation assays. Cell apoptosis and cell cycle distribution were investigated by flow cytometry. Results: In human lung and cervical cancer cell lines, Niraparib combined with radiation therapy significantly inhibited cell proliferation. The proportion of apoptotic cells in cell lines treated with Niraparib plus radiation (the combination group) was significantly higher compared with control, radiation, and Niraparib groups (P < 0.05). Additionally, the proportion of A549 cells in the G2/M phase was significantly increased in the combination group compared with the radiation group (P < 0.05). Conclusion: PARP inhibitor Niraparib increases the radiosensitivity of tumorcells, promotes their apoptosis, and induces cell cycle redistribution. The possible mechanism is associated with the inhibition of radiation-induced DNA damage repair.http://www.sciencedirect.com/science/article/pii/S2666555723000229DNA damage repairPARP inhibitorRadiation therapyRadiosensitivityTumor |
| spellingShingle | Yue Feng Shiyi Pi Yuwei Li Yu Zhang Hongwei Zeng E. Xiangyu Yang Zhang Jingping Yu Effects of the PARP inhibitor Niraparib on the radiosensitivity of human lung and cervical cancer cells Radiation Medicine and Protection DNA damage repair PARP inhibitor Radiation therapy Radiosensitivity Tumor |
| title | Effects of the PARP inhibitor Niraparib on the radiosensitivity of human lung and cervical cancer cells |
| title_full | Effects of the PARP inhibitor Niraparib on the radiosensitivity of human lung and cervical cancer cells |
| title_fullStr | Effects of the PARP inhibitor Niraparib on the radiosensitivity of human lung and cervical cancer cells |
| title_full_unstemmed | Effects of the PARP inhibitor Niraparib on the radiosensitivity of human lung and cervical cancer cells |
| title_short | Effects of the PARP inhibitor Niraparib on the radiosensitivity of human lung and cervical cancer cells |
| title_sort | effects of the parp inhibitor niraparib on the radiosensitivity of human lung and cervical cancer cells |
| topic | DNA damage repair PARP inhibitor Radiation therapy Radiosensitivity Tumor |
| url | http://www.sciencedirect.com/science/article/pii/S2666555723000229 |
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