Assay method for mitochondrial sterol 27-hydroxylase with 7α-hydroxy-4-cholesten-3-one as a substrate in the rat liver

Assay method for mitochondrial sterol 27-hydroxylase with 7α-hydroxy-4-cholesten-3-one as a substrate in the rat liver

Mitochondrial sterol 27-hydroxylase (EC 1.14.13.15) is an important enzyme, not only in the formation of bile acids from cholesterol intermediates in the liver but also in the removal of cholesterol by side chain hydroxylation in extrahepatic tissues. The enzyme has been assayed by complicated metho...

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Bibliographic Details
Main Authors: Yoshikazu Ota, Tada-Aki Eto, Shun-Ichi Tanaka, Hideto Sueta, Hironori Shiotsuki, Yorio Maeda, Mizuho Une, Kazuo Chijiiwa
Format: Article
Language:English
Published: Elsevier 2003-12-01
Series:Journal of Lipid Research
Subjects:
adrenodoxin
NADPH-adrenodoxin reductase
7α,27-dihydroxy-4-cholesten-3-one
high-performance liquid chromatography
solubilization
CYP27A1
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520319593

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http://www.sciencedirect.com/science/article/pii/S0022227520319593

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