Improvement of Fab expression by screening combinatorial synonymous signal sequence libraries

Improvement of Fab expression by screening combinatorial synonymous signal sequence libraries

Abstract Background Antibody fragments can be expressed in Escherichia coli, where they are commonly directed to the periplasm via Sec pathway to enable disulphide bridge formations and correct folding. In order to transport antibody fragments to the periplasmic space via Sec pathway, they are equip...

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Bibliographic Details
Main Authors: Antti Kulmala, Tuomas Huovinen, Urpo Lamminmäki
Format: Article
Language:English
Published: BMC 2019-09-01
Series:Microbial Cell Factories
Subjects:
Codon usage
Fab fragment
Heterologous expression
Escherichia coli
PelB
Online Access:http://link.springer.com/article/10.1186/s12934-019-1210-1

Internet

http://link.springer.com/article/10.1186/s12934-019-1210-1

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