Cryopreservation of rat embryos at all developmental stages by small-volume vitrification procedure and rapid warming in cryotubes
Abstract Intracellular ice formation during cryopreservation is lethal to the cell, including during warming. Here, we examined the effect of sample volume and warming rate on the cryopreservation success of 1-cell rat embryos based on successful development into blastocysts in vitro and to term in...
Main Authors: | , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2023-11-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-023-47394-0 |