Cryopreservation of rat embryos at all developmental stages by small-volume vitrification procedure and rapid warming in cryotubes

Abstract Intracellular ice formation during cryopreservation is lethal to the cell, including during warming. Here, we examined the effect of sample volume and warming rate on the cryopreservation success of 1-cell rat embryos based on successful development into blastocysts in vitro and to term in...

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Bibliographic Details
Main Authors: Shinsuke Seki, Toshiaki Kawabe, Wataru Yamazaki, Kazuaki Matsumura, Takanori Oikawa, Takahiro Obata, Misako Higashiya, Megumi Yano, Tomoo Eto
Format: Article
Language:English
Published: Nature Portfolio 2023-11-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-47394-0