Start codon disruption with CRISPR/Cas9 prevents murine Fuchs’ endothelial corneal dystrophy

Start codon disruption with CRISPR/Cas9 prevents murine Fuchs’ endothelial corneal dystrophy

A missense mutation of collagen type VIII alpha 2 chain (COL8A2) gene leads to early-onset Fuchs’ endothelial corneal dystrophy (FECD), which progressively impairs vision through the loss of corneal endothelial cells. We demonstrate that CRISPR/Cas9-based postnatal gene editing achieves structural a...

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Bibliographic Details
Main Authors: Hironori Uehara, Xiaohui Zhang, Felipe Pereira, Siddharth Narendran, Susie Choi, Sai Bhuvanagiri, Jinlu Liu, Sangeetha Ravi Kumar, Austin Bohner, Lara Carroll, Bonnie Archer, Yue Zhang, Wei Liu, Guangping Gao, Jayakrishna Ambati, Albert S Jun, Balamurali K Ambati
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2021-06-01
Series:eLife
Subjects:
Fuchs endothelial corneal dystrophy
CRISPR/Cas9
Adenovirus
off-target analysis of CRISPR/Cas9
Online Access:https://elifesciences.org/articles/55637

Internet

https://elifesciences.org/articles/55637

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