Strategies for Efficient Gene Editing in Protoplasts of Solanum tuberosum Theme: Determining gRNA Efficiency Design by Utilizing Protoplast (Research)

Strategies for Efficient Gene Editing in Protoplasts of Solanum tuberosum Theme: Determining gRNA Efficiency Design by Utilizing Protoplast (Research)

Potato (Solanum tuberosum) is a highly diverse tetraploid crop. Elite cultivars are extremely heterozygous with a high prevalence of small length polymorphisms (indels) and single nucleotide polymorphisms (SNPs) within and between cultivars, which must be considered in CRISPR/Cas gene editing strate...

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Bibliographic Details
Main Authors: Frida Meijer Carlsen, Ida Elisabeth Johansen, Zhang Yang, Ying Liu, Ida Nøhr Westberg, Nam Phuong Kieu, Bodil Jørgensen, Marit Lenman, Erik Andreasson, Kåre Lehmann Nielsen, Andreas Blennow, Bent Larsen Petersen
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-01-01
Series:Frontiers in Genome Editing
Subjects:
ribonucleoprotein/gRNA design
gene editing
protoplast
CRISPR/Cas
complex genome
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2021.795644/full

Internet

https://www.frontiersin.org/articles/10.3389/fgeed.2021.795644/full

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