Influence of Long-Term Agar-Slant Preservation at 4 °C on the Recombinant Enzyme Activity of Engineered Yeast

Influence of Long-Term Agar-Slant Preservation at 4 °C on the Recombinant Enzyme Activity of Engineered Yeast

Strain preservation to maintain stable vitality and the recombinant enzyme activity plays a crucial role in industrial fermentation. A <i>Pichia pastoris</i> strain is routinely stored at −80 °C in a glycerol vial and activated on an antibiotic-containing YPD agar plate before being used...

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Bibliographic Details
Main Authors: Xiao Liang, Ting Gong, Jing-Jing Chen, Tian-Jiao Chen, Jin-Ling Yang, Ping Zhu
Format: Article
Language:English
Published: MDPI AG 2023-01-01
Series:Fermentation
Subjects:
strain preservation
<i>Pichia pastori</i>
industrial fermentation
low temperature storage
vitality
production efficiency
Online Access:https://www.mdpi.com/2311-5637/9/2/104

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https://www.mdpi.com/2311-5637/9/2/104

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