In Vitro Evaluation of Wood Vinegar (Pyroligneous Acid) VOCs Inhibitory Effect against a Fungus-like Microorganism <i>Ovatisporangium</i> (<i>Phytopythium</i>) Isolate Recovered from Tomato Fields in Iran
Crop diseases and agricultural pests and pathogens are causing huge economic losses. The actual means for dealing with them involve the use of damaging chemical pesticides that harm the environment, threaten biodiversity, and undermine human health. This research was aimed at developing an environme...
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MDPI AG
2022-07-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/7/1609 |
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| author | Ali Chenari Bouket Abolfazl Narmani Alireza Tavasolee Ghorban Elyasi Akbar Abdi Shahram Naeimi Kasra Sharifi Tomasz Oszako Faizah N. Alenezi Lassaad Belbahri |
| author_facet | Ali Chenari Bouket Abolfazl Narmani Alireza Tavasolee Ghorban Elyasi Akbar Abdi Shahram Naeimi Kasra Sharifi Tomasz Oszako Faizah N. Alenezi Lassaad Belbahri |
| author_sort | Ali Chenari Bouket |
| collection | DOAJ |
| description | Crop diseases and agricultural pests and pathogens are causing huge economic losses. The actual means for dealing with them involve the use of damaging chemical pesticides that harm the environment, threaten biodiversity, and undermine human health. This research was aimed at developing an environmentally friendly means to cope with emerging oomycete disease from tomato fields in the province of East-Azerbaijan. The oomycete disease causal agent was isolated and identified as <i>Ovatisporangium</i> sp. using a combination of morphological features and molecular methods. Six wood vinegars (pyroligneous acid) belonging to pine, pomegranate, pistachio, almond, walnut, and cypress were produced during this study and examined against <i>Ovatisporangium</i> sp. Their inhibition of volatile metabolites (VOCs) using different dilutions (1, 1/2, 1/4, 1/8, and 1/10) was assessed against the mycelial growth of <i>Ovatisporangium</i> sp. In vitro analysis demonstrated that pistachio, cypress, and almond dilution 1 (D 1) wood vinegar VOCs had the ability to stop the mycelial growth of <i>Ovatisporangium</i> sp. All other treatments including pine, walnut, and pomegranate with relevant dilutions significantly reduced the mycelial growth of <i>Ovatisporangium</i> sp. compared with the control (<i>p</i> ≤ 0.05). Wood vinegar is therefore a potent means to cope with pathogenic infections and allows plant protection against oomycete diseases. |
| first_indexed | 2024-03-09T10:24:41Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T10:24:41Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-c9fd9c487860472496cb928e82e2b9372023-12-01T21:47:18ZengMDPI AGAgronomy2073-43952022-07-01127160910.3390/agronomy12071609In Vitro Evaluation of Wood Vinegar (Pyroligneous Acid) VOCs Inhibitory Effect against a Fungus-like Microorganism <i>Ovatisporangium</i> (<i>Phytopythium</i>) Isolate Recovered from Tomato Fields in IranAli Chenari Bouket0Abolfazl Narmani1Alireza Tavasolee2Ghorban Elyasi3Akbar Abdi4Shahram Naeimi5Kasra Sharifi6Tomasz Oszako7Faizah N. Alenezi8Lassaad Belbahri9East Azarbaijan Agricultural and Natural Resources Research and Education Centre, Plant Protection Research Department, Agricultural Research, Education and Extension Organization (AREEO), Tabriz 5355179854, IranDepartment of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz 5166614766, IranEast Azarbaijan Agricultural and Natural Resources Research and Education Center, Soil and Water Research Department, Agricultural Research, Education and Extension Organization (AREEO), Tabriz 5355179854, IranEast Azarbaijan Agricultural and Natural Resources Research and Education Center, Animal Sciences Research Department, Agricultural Research, Education and Extension Organization (AREEO), Tabriz 5355179854, IranEast Azarbaijan Agricultural and Natural Resources Research and Education Center, Natural Resources Research Department, Agricultural Research, Education and Extension Organization (AREEO), Tabriz 5355179854, IranBiological Control Research Department, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran 1985813111, IranPlant Disease Research Department, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran 1985813111, IranDepartment of Forest Protection, Forest Research Institute in Sekocin Stary, 05-090 Raszyn, PolandMarine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Old Aberdeen, Scotland AB24 3UE, UKLaboratory of Soil Biology, University of Neuchatel, 2000 Neuchatel, SwitzerlandCrop diseases and agricultural pests and pathogens are causing huge economic losses. The actual means for dealing with them involve the use of damaging chemical pesticides that harm the environment, threaten biodiversity, and undermine human health. This research was aimed at developing an environmentally friendly means to cope with emerging oomycete disease from tomato fields in the province of East-Azerbaijan. The oomycete disease causal agent was isolated and identified as <i>Ovatisporangium</i> sp. using a combination of morphological features and molecular methods. Six wood vinegars (pyroligneous acid) belonging to pine, pomegranate, pistachio, almond, walnut, and cypress were produced during this study and examined against <i>Ovatisporangium</i> sp. Their inhibition of volatile metabolites (VOCs) using different dilutions (1, 1/2, 1/4, 1/8, and 1/10) was assessed against the mycelial growth of <i>Ovatisporangium</i> sp. In vitro analysis demonstrated that pistachio, cypress, and almond dilution 1 (D 1) wood vinegar VOCs had the ability to stop the mycelial growth of <i>Ovatisporangium</i> sp. All other treatments including pine, walnut, and pomegranate with relevant dilutions significantly reduced the mycelial growth of <i>Ovatisporangium</i> sp. compared with the control (<i>p</i> ≤ 0.05). Wood vinegar is therefore a potent means to cope with pathogenic infections and allows plant protection against oomycete diseases.https://www.mdpi.com/2073-4395/12/7/1609<i>Ovatisporangium</i> (<i>Phytopythium</i>)in vitropyroligneous acidVOCswood vinegar |
| spellingShingle | Ali Chenari Bouket Abolfazl Narmani Alireza Tavasolee Ghorban Elyasi Akbar Abdi Shahram Naeimi Kasra Sharifi Tomasz Oszako Faizah N. Alenezi Lassaad Belbahri In Vitro Evaluation of Wood Vinegar (Pyroligneous Acid) VOCs Inhibitory Effect against a Fungus-like Microorganism <i>Ovatisporangium</i> (<i>Phytopythium</i>) Isolate Recovered from Tomato Fields in Iran Agronomy <i>Ovatisporangium</i> (<i>Phytopythium</i>) in vitro pyroligneous acid VOCs wood vinegar |
| title | In Vitro Evaluation of Wood Vinegar (Pyroligneous Acid) VOCs Inhibitory Effect against a Fungus-like Microorganism <i>Ovatisporangium</i> (<i>Phytopythium</i>) Isolate Recovered from Tomato Fields in Iran |
| title_full | In Vitro Evaluation of Wood Vinegar (Pyroligneous Acid) VOCs Inhibitory Effect against a Fungus-like Microorganism <i>Ovatisporangium</i> (<i>Phytopythium</i>) Isolate Recovered from Tomato Fields in Iran |
| title_fullStr | In Vitro Evaluation of Wood Vinegar (Pyroligneous Acid) VOCs Inhibitory Effect against a Fungus-like Microorganism <i>Ovatisporangium</i> (<i>Phytopythium</i>) Isolate Recovered from Tomato Fields in Iran |
| title_full_unstemmed | In Vitro Evaluation of Wood Vinegar (Pyroligneous Acid) VOCs Inhibitory Effect against a Fungus-like Microorganism <i>Ovatisporangium</i> (<i>Phytopythium</i>) Isolate Recovered from Tomato Fields in Iran |
| title_short | In Vitro Evaluation of Wood Vinegar (Pyroligneous Acid) VOCs Inhibitory Effect against a Fungus-like Microorganism <i>Ovatisporangium</i> (<i>Phytopythium</i>) Isolate Recovered from Tomato Fields in Iran |
| title_sort | in vitro evaluation of wood vinegar pyroligneous acid vocs inhibitory effect against a fungus like microorganism i ovatisporangium i i phytopythium i isolate recovered from tomato fields in iran |
| topic | <i>Ovatisporangium</i> (<i>Phytopythium</i>) in vitro pyroligneous acid VOCs wood vinegar |
| url | https://www.mdpi.com/2073-4395/12/7/1609 |
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