ACIDES: on-line monitoring of forward genetic screens for protein engineering

Abstract Forward genetic screens of mutated variants are a versatile strategy for protein engineering and investigation, which has been successfully applied to various studies like directed evolution (DE) and deep mutational scanning (DMS). While next-generation sequencing can track millions of vari...

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Bibliographic Details
Main Authors: Takahiro Nemoto, Tommaso Ocari, Arthur Planul, Muge Tekinsoy, Emilia A. Zin, Deniz Dalkara, Ulisse Ferrari
Format: Article
Language:English
Published: Nature Portfolio 2023-12-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-023-43967-9