Metabolites of De Novo Purine Synthesis: Metabolic Regulators and Cytotoxic Compounds
Cytotoxicity of de novo purine synthesis (DNPS) metabolites is critical to the pathogenesis of three known and one putative autosomal recessive disorder affecting DNPS. These rare disorders are caused by biallelic mutations in the DNPS genes phosphoribosylformylglycineamidine synthase (PFAS), phosph...
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2022-12-01
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| author | Olga Souckova Vaclava Skopova Veronika Baresova David Sedlak Anthony J. Bleyer Stanislav Kmoch Marie Zikanova |
| author_facet | Olga Souckova Vaclava Skopova Veronika Baresova David Sedlak Anthony J. Bleyer Stanislav Kmoch Marie Zikanova |
| author_sort | Olga Souckova |
| collection | DOAJ |
| description | Cytotoxicity of de novo purine synthesis (DNPS) metabolites is critical to the pathogenesis of three known and one putative autosomal recessive disorder affecting DNPS. These rare disorders are caused by biallelic mutations in the DNPS genes phosphoribosylformylglycineamidine synthase (PFAS), phosphoribosylaminoimidazolecarboxylase/phosphoribosylaminoimidazolesuccinocarboxamide synthase (PAICS), adenylosuccinate lyase (ADSL), and aminoimidazole carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase (ATIC) and are clinically characterized by developmental abnormalities, psychomotor retardation, and nonspecific neurological impairment. At a biochemical level, loss of function of specific mutated enzymes results in elevated levels of DNPS ribosides in body fluids. The main pathogenic effect is attributed to the accumulation of DNPS ribosides, which are postulated to be toxic to the organism. Therefore, we decided to characterize the uptake and flux of several DNPS metabolites in HeLa cells and the impact of DNPS metabolites to viability of cancer cell lines and primary skin fibroblasts. We treated cells with DNPS metabolites and followed their flux in purine synthesis and degradation. In this study, we show for the first time the transport of formylglycinamide ribotide (FGAR), aminoimidazole ribotide (AIR), succinylaminoimidazolecarboxamide ribotide (SAICAR), and aminoimidazolecarboxamide ribotide (AICAR) into cells and their flux in DNPS and the degradation pathway. We found diminished cell viability mostly in the presence of FGAR and AIR. Our results suggest that direct cellular toxicity of DNPS metabolites may not be the primary pathogenetic mechanism in these disorders. |
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| spelling | doaj.art-ca41efae37a14af5b665d8b05d9bd17a2023-11-24T16:37:34ZengMDPI AGMetabolites2218-19892022-12-011212121010.3390/metabo12121210Metabolites of De Novo Purine Synthesis: Metabolic Regulators and Cytotoxic CompoundsOlga Souckova0Vaclava Skopova1Veronika Baresova2David Sedlak3Anthony J. Bleyer4Stanislav Kmoch5Marie Zikanova6Department of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08 Prague, Czech RepublicDepartment of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08 Prague, Czech RepublicDepartment of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08 Prague, Czech RepublicCZ-OPENSCREEN: National Infrastructure for Chemical Biology, Institute of Molecular Genetics, Czech Academy of Sciences, 142 00 Prague, Czech RepublicDepartment of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08 Prague, Czech RepublicDepartment of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08 Prague, Czech RepublicDepartment of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08 Prague, Czech RepublicCytotoxicity of de novo purine synthesis (DNPS) metabolites is critical to the pathogenesis of three known and one putative autosomal recessive disorder affecting DNPS. These rare disorders are caused by biallelic mutations in the DNPS genes phosphoribosylformylglycineamidine synthase (PFAS), phosphoribosylaminoimidazolecarboxylase/phosphoribosylaminoimidazolesuccinocarboxamide synthase (PAICS), adenylosuccinate lyase (ADSL), and aminoimidazole carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase (ATIC) and are clinically characterized by developmental abnormalities, psychomotor retardation, and nonspecific neurological impairment. At a biochemical level, loss of function of specific mutated enzymes results in elevated levels of DNPS ribosides in body fluids. The main pathogenic effect is attributed to the accumulation of DNPS ribosides, which are postulated to be toxic to the organism. Therefore, we decided to characterize the uptake and flux of several DNPS metabolites in HeLa cells and the impact of DNPS metabolites to viability of cancer cell lines and primary skin fibroblasts. We treated cells with DNPS metabolites and followed their flux in purine synthesis and degradation. In this study, we show for the first time the transport of formylglycinamide ribotide (FGAR), aminoimidazole ribotide (AIR), succinylaminoimidazolecarboxamide ribotide (SAICAR), and aminoimidazolecarboxamide ribotide (AICAR) into cells and their flux in DNPS and the degradation pathway. We found diminished cell viability mostly in the presence of FGAR and AIR. Our results suggest that direct cellular toxicity of DNPS metabolites may not be the primary pathogenetic mechanism in these disorders.https://www.mdpi.com/2218-1989/12/12/1210purine synthesisPFASPAICSADSLATICcytotoxicity |
| spellingShingle | Olga Souckova Vaclava Skopova Veronika Baresova David Sedlak Anthony J. Bleyer Stanislav Kmoch Marie Zikanova Metabolites of De Novo Purine Synthesis: Metabolic Regulators and Cytotoxic Compounds Metabolites purine synthesis PFAS PAICS ADSL ATIC cytotoxicity |
| title | Metabolites of De Novo Purine Synthesis: Metabolic Regulators and Cytotoxic Compounds |
| title_full | Metabolites of De Novo Purine Synthesis: Metabolic Regulators and Cytotoxic Compounds |
| title_fullStr | Metabolites of De Novo Purine Synthesis: Metabolic Regulators and Cytotoxic Compounds |
| title_full_unstemmed | Metabolites of De Novo Purine Synthesis: Metabolic Regulators and Cytotoxic Compounds |
| title_short | Metabolites of De Novo Purine Synthesis: Metabolic Regulators and Cytotoxic Compounds |
| title_sort | metabolites of de novo purine synthesis metabolic regulators and cytotoxic compounds |
| topic | purine synthesis PFAS PAICS ADSL ATIC cytotoxicity |
| url | https://www.mdpi.com/2218-1989/12/12/1210 |
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