Electroporation of CRISPR/Cas9 Targeting Neurogenin 3 (NGN3) in Porcine Embryos and Its Effects on Mosaicism and Off-Target Effects by Next Generation Sequencing (NGS)

Electroporation of CRISPR/Cas9 Targeting Neurogenin 3 (NGN3) in Porcine Embryos and Its Effects on Mosaicism and Off-Target Effects by Next Generation Sequencing (NGS)

Introduction: CRISPR/Cas9-mediated editing of embryos through microinjection is limited in efficiency because of high rates of mosaicism and off-target mutations. Electroporation has been proposed as an easier and faster procedure compared to single embryo injection. Methods: Using a synthetic guide...

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Bibliographic Details
Main Authors: Insung Park, Sergio Navarro-Serna, Raquel M. Pinho, Trish Berger, Elizabeth A. Maga, Joaqu?n Gadea, Seung K. Kim, Pablo J. Ross
Format: Article
Language:English
Published: Mary Ann Liebert 2024-01-01
Series:Re:GEN Open
Subjects:
Pig embryos
CRISPR/Cas9 electroporation
mosaicism
off-target effects
Online Access:https://www.liebertpub.com/doi/full/10.1089/REGEN.2023.0021

Internet

https://www.liebertpub.com/doi/full/10.1089/REGEN.2023.0021

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