A doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editing
Achieving spatial control of gene expression is important. Here the authors report an optimised photoactivatable Cre recombinase system, doxycycline- and light-inducible Cre recombinase (DiLiCre), and generate a DiLiCre mouse line which they use for mutagenesis in vivo and positional cell-tracing.
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-33863-z |
| _version_ | 1797991647959580672 |
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| author | Miguel Vizoso Colin E. J. Pritchard Lorenzo Bombardelli Bram van den Broek Paul Krimpenfort Roderick L. Beijersbergen Kees Jalink Jacco van Rheenen |
| author_facet | Miguel Vizoso Colin E. J. Pritchard Lorenzo Bombardelli Bram van den Broek Paul Krimpenfort Roderick L. Beijersbergen Kees Jalink Jacco van Rheenen |
| author_sort | Miguel Vizoso |
| collection | DOAJ |
| description | Achieving spatial control of gene expression is important. Here the authors report an optimised photoactivatable Cre recombinase system, doxycycline- and light-inducible Cre recombinase (DiLiCre), and generate a DiLiCre mouse line which they use for mutagenesis in vivo and positional cell-tracing. |
| first_indexed | 2024-04-11T08:55:28Z |
| format | Article |
| id | doaj.art-ca99867f51bf4b11a30935fbe673b977 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-11T08:55:28Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-ca99867f51bf4b11a30935fbe673b9772022-12-22T04:33:19ZengNature PortfolioNature Communications2041-17232022-10-0113111510.1038/s41467-022-33863-zA doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editingMiguel Vizoso0Colin E. J. Pritchard1Lorenzo Bombardelli2Bram van den Broek3Paul Krimpenfort4Roderick L. Beijersbergen5Kees Jalink6Jacco van Rheenen7Department of Molecular Pathology, Oncode Institute, Netherlands Cancer InstituteMouse Clinic for Cancer and Aging, The Netherlands Cancer InstituteDivision of Molecular Carcinogenesis and Oncode Institute, Netherlands Cancer InstituteCell Biophysics Group, Department of Cell Biology, The Netherlands Cancer InstituteMouse Clinic for Cancer and Aging, The Netherlands Cancer InstituteDivision of Molecular Carcinogenesis and Oncode Institute, Netherlands Cancer InstituteCell Biophysics Group, Department of Cell Biology, The Netherlands Cancer InstituteDepartment of Molecular Pathology, Oncode Institute, Netherlands Cancer InstituteAchieving spatial control of gene expression is important. Here the authors report an optimised photoactivatable Cre recombinase system, doxycycline- and light-inducible Cre recombinase (DiLiCre), and generate a DiLiCre mouse line which they use for mutagenesis in vivo and positional cell-tracing.https://doi.org/10.1038/s41467-022-33863-z |
| spellingShingle | Miguel Vizoso Colin E. J. Pritchard Lorenzo Bombardelli Bram van den Broek Paul Krimpenfort Roderick L. Beijersbergen Kees Jalink Jacco van Rheenen A doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editing Nature Communications |
| title | A doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editing |
| title_full | A doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editing |
| title_fullStr | A doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editing |
| title_full_unstemmed | A doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editing |
| title_short | A doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editing |
| title_sort | doxycycline and light inducible cre recombinase mouse model for optogenetic genome editing |
| url | https://doi.org/10.1038/s41467-022-33863-z |
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