H3K36 methyltransferase NSD1 is essential for normal B1 and B2 cell development and germinal center formation
B cells, which consist of two well-defined populations: B1 and B2 cells, which can produce antibodies that are essential for host protection against infections, through virus neutralization, opsonization and antibody-dependent cellular cytotoxicity. Epigenetic modifications, such as DNA methylation...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.959021/full |
| _version_ | 1811213855002460160 |
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| author | Sulan Zhai Min Cao Han Zhou Han Zhou Huamin Zhu Tongchang Xu Yuliang Wang Xiaoming Wang Xiaoming Wang Xiaoming Wang Zhenming Cai |
| author_facet | Sulan Zhai Min Cao Han Zhou Han Zhou Huamin Zhu Tongchang Xu Yuliang Wang Xiaoming Wang Xiaoming Wang Xiaoming Wang Zhenming Cai |
| author_sort | Sulan Zhai |
| collection | DOAJ |
| description | B cells, which consist of two well-defined populations: B1 and B2 cells, which can produce antibodies that are essential for host protection against infections, through virus neutralization, opsonization and antibody-dependent cellular cytotoxicity. Epigenetic modifications, such as DNA methylation and histone modification could regulate immune cell differentiation and functions. In this study, we found a significant reduction of GC response in the B cell specific knockout of H3K36 methyltransferase NSD1 (Mb1-Cre+ NSD1fl/fl, NSD1B KO) mice compared with the wildtype control (Mb1-Cre+ NSD1+/+, NSD1B WT). We also demonstrated reduced production of high-affinity antibody, but increased production of low-affinity antibody in the NSD1B KO mice. Further analysis revealed that loss of NSD1 promoted the development of B1 cells by increasing the expression of Rap1b and Arid3a. In conclusion, our data suggest that NSD1 plays an important role in regulation the development of B1 and B2 cells, and the process of germinal center formation and high-affinity antibody production. |
| first_indexed | 2024-04-12T05:53:15Z |
| format | Article |
| id | doaj.art-caeba1ad6f62450190cca9466f411b5a |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-12T05:53:15Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-caeba1ad6f62450190cca9466f411b5a2022-12-22T03:45:15ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-11-011310.3389/fimmu.2022.959021959021H3K36 methyltransferase NSD1 is essential for normal B1 and B2 cell development and germinal center formationSulan Zhai0Min Cao1Han Zhou2Han Zhou3Huamin Zhu4Tongchang Xu5Yuliang Wang6Xiaoming Wang7Xiaoming Wang8Xiaoming Wang9Zhenming Cai10Department of Immunology, Key Laboratory of Immune Microenvironment and Diseases, Nanjing Medical University, Nanjing, ChinaDepartment of Immunology, Key Laboratory of Immune Microenvironment and Diseases, Nanjing Medical University, Nanjing, ChinaDepartment of Immunology, Key Laboratory of Immune Microenvironment and Diseases, Nanjing Medical University, Nanjing, ChinaReproductive Medicine Centre, Changzhou No. 2 People’s Hospital, The Affiliated Hospital of Nanjing Medical University, Changzhou, ChinaDepartment of Immunology, Key Laboratory of Immune Microenvironment and Diseases, Nanjing Medical University, Nanjing, ChinaDepartment of Immunology, Key Laboratory of Immune Microenvironment and Diseases, Nanjing Medical University, Nanjing, ChinaDepartment of Immunology, Key Laboratory of Immune Microenvironment and Diseases, Nanjing Medical University, Nanjing, ChinaDepartment of Immunology, Key Laboratory of Immune Microenvironment and Diseases, Nanjing Medical University, Nanjing, ChinaState Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, ChinaNational Health Commission (NHC) Key Laboratory of Antibody Technique, Nanjing Medical University, Nanjing, ChinaDepartment of Immunology, Key Laboratory of Immune Microenvironment and Diseases, Nanjing Medical University, Nanjing, ChinaB cells, which consist of two well-defined populations: B1 and B2 cells, which can produce antibodies that are essential for host protection against infections, through virus neutralization, opsonization and antibody-dependent cellular cytotoxicity. Epigenetic modifications, such as DNA methylation and histone modification could regulate immune cell differentiation and functions. In this study, we found a significant reduction of GC response in the B cell specific knockout of H3K36 methyltransferase NSD1 (Mb1-Cre+ NSD1fl/fl, NSD1B KO) mice compared with the wildtype control (Mb1-Cre+ NSD1+/+, NSD1B WT). We also demonstrated reduced production of high-affinity antibody, but increased production of low-affinity antibody in the NSD1B KO mice. Further analysis revealed that loss of NSD1 promoted the development of B1 cells by increasing the expression of Rap1b and Arid3a. In conclusion, our data suggest that NSD1 plays an important role in regulation the development of B1 and B2 cells, and the process of germinal center formation and high-affinity antibody production.https://www.frontiersin.org/articles/10.3389/fimmu.2022.959021/fullNSD1B2 cellsgerminal centerantibodyB1 cells |
| spellingShingle | Sulan Zhai Min Cao Han Zhou Han Zhou Huamin Zhu Tongchang Xu Yuliang Wang Xiaoming Wang Xiaoming Wang Xiaoming Wang Zhenming Cai H3K36 methyltransferase NSD1 is essential for normal B1 and B2 cell development and germinal center formation Frontiers in Immunology NSD1 B2 cells germinal center antibody B1 cells |
| title | H3K36 methyltransferase NSD1 is essential for normal B1 and B2 cell development and germinal center formation |
| title_full | H3K36 methyltransferase NSD1 is essential for normal B1 and B2 cell development and germinal center formation |
| title_fullStr | H3K36 methyltransferase NSD1 is essential for normal B1 and B2 cell development and germinal center formation |
| title_full_unstemmed | H3K36 methyltransferase NSD1 is essential for normal B1 and B2 cell development and germinal center formation |
| title_short | H3K36 methyltransferase NSD1 is essential for normal B1 and B2 cell development and germinal center formation |
| title_sort | h3k36 methyltransferase nsd1 is essential for normal b1 and b2 cell development and germinal center formation |
| topic | NSD1 B2 cells germinal center antibody B1 cells |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.959021/full |
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