Detection of gene cis-regulatory element perturbations in single-cell transcriptomes.

Detection of gene cis-regulatory element perturbations in single-cell transcriptomes.

We introduce poly-adenine CRISPR gRNA-based single-cell RNA-sequencing (pAC-Seq), a method that enables the direct observation of guide RNAs (gRNAs) in scRNA-seq. We use pAC-Seq to assess the phenotypic consequences of CRISPR/Cas9 based alterations of gene cis-regulatory regions. We show that pAC-Se...

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Bibliographic Details
Main Authors: Grace Hui Ting Yeo, Oscar Juez, Qing Chen, Budhaditya Banerjee, Lendy Chu, Max W Shen, May Sabry, Ive Logister, Richard I Sherwood, David K Gifford
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2021-03-01
Series:PLoS Computational Biology
Online Access:https://doi.org/10.1371/journal.pcbi.1008789

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https://doi.org/10.1371/journal.pcbi.1008789

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