Genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements

Genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements

Here, the authors show that reticulocytes derived from immortalized erythroblasts support invasion and development of Plasmodium falciparum and use CRISPR-mediated gene knockout and complementation of an invasion receptor to demonstrate utility of this model system for research in malaria invasion.

Bibliographic Details
Main Authors: Timothy J. Satchwell, Katherine E. Wright, Katy L. Haydn-Smith, Fernando Sánchez-Román Terán, Pedro L. Moura, Joseph Hawksworth, Jan Frayne, Ashley M. Toye, Jake Baum
Format: Article
Language:English
Published: Nature Portfolio 2019-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-11790-w

Internet

https://doi.org/10.1038/s41467-019-11790-w

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