A multiplex RPA coupled with CRISPR-Cas12a system for rapid and cost-effective identification of carbapenem-resistant Acinetobacter baumannii

A multiplex RPA coupled with CRISPR-Cas12a system for rapid and cost-effective identification of carbapenem-resistant Acinetobacter baumannii

BackgroundCarbapenem-resistant Acinetobacter baumannii (CRAB) poses a severe nosocomial threat, prompting a need for efficient detection methods. Traditional approaches, such as bacterial culture and PCR, are time-consuming and cumbersome. The CRISPR-based gene editing system offered a potential app...

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Bibliographic Details
Main Authors: Zihan Zhou, Lina Liang, Chuan Liao, Lele Pan, Chunfang Wang, Jiangmei Ma, Xueli Yi, Meiying Tan, Xuebin Li, Guijiang Wei
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-03-01
Series:Frontiers in Microbiology
Subjects:
Acinetobacter baumannii
recombinase polymerase amplification
CRISPR-Cas12a
nucleic acid detection
point-of-care testing
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2024.1359976/full

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https://www.frontiersin.org/articles/10.3389/fmicb.2024.1359976/full

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