Supplemental <i>Aspergillus</i> Lipase and Protease Preparations Display Powerful Bifidogenic Effects and Modulate the Gut Microbiota Community of Rats
<i>Aspergillus</i>-derived protease and lipase, which are involved in the production of <i>Aspergillus</i>-fermented foods, are consumed as digestive enzyme supplements. A marked bifidogenic effect of supplemental <i>Aspergillus</i> protease preparation (AP) in ra...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/7/4/294 |
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| author | Yongshou Yang Thanutchaporn Kumrungsee Norihisa Kato Shinji Fukuda Manabu Kuroda Shotaro Yamaguchi |
| author_facet | Yongshou Yang Thanutchaporn Kumrungsee Norihisa Kato Shinji Fukuda Manabu Kuroda Shotaro Yamaguchi |
| author_sort | Yongshou Yang |
| collection | DOAJ |
| description | <i>Aspergillus</i>-derived protease and lipase, which are involved in the production of <i>Aspergillus</i>-fermented foods, are consumed as digestive enzyme supplements. A marked bifidogenic effect of supplemental <i>Aspergillus</i> protease preparation (AP) in rats fed with a high-fat diet was identified. This study was conducted to examine whether the consumption of <i>Aspergillus</i>-derived lipase exerts similar bifidogenic effect. Rats were fed diets supplemented with either an <i>Aspergillus</i>-derived lipase preparation (AL) or AP at 0.1% for two weeks. 16S rRNA gene sequencing analysis indicated that supplemental AL and AP markedly influenced cecal microbial community. At the phylum level, treatment with AL and AP resulted in a lower relative abundance of Firmicutes and Bacteroidetes, but a higher relative abundance of Actinobacteria and Proteobacteria than the control rats (<i>p</i> < 0.05). At the genus level, AL and AP remarkedly elevated the relative abundances of <i>Bifidobacterium</i>, <i>Collinsella</i>, and <i>Enterococcus</i>, but significantly reduced those of <i>Oscillospira</i>, <i>Dorea</i>, and <i>Coprobacillus</i> (<i>p</i> < 0.05). These modulations were similar to those reported by several studies with typical prebiotic oligosaccharides. Notably, the bifidogenic effect of AL was much greater than that of AP. Our results show that the two different <i>Aspergillus</i>-derived preparations, AL and AP, have strong bifidogenic effects and can change the microbiota’s composition. |
| first_indexed | 2024-03-10T04:10:30Z |
| format | Article |
| id | doaj.art-cb544de6541a4d69a2543c6b5f0cecec |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T04:10:30Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-cb544de6541a4d69a2543c6b5f0cecec2023-11-23T08:13:37ZengMDPI AGFermentation2311-56372021-12-017429410.3390/fermentation7040294Supplemental <i>Aspergillus</i> Lipase and Protease Preparations Display Powerful Bifidogenic Effects and Modulate the Gut Microbiota Community of RatsYongshou Yang0Thanutchaporn Kumrungsee1Norihisa Kato2Shinji Fukuda3Manabu Kuroda4Shotaro Yamaguchi5School of Life Sciences, Anhui University, Hefei 230036, ChinaGraduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima 739-8528, JapanGraduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima 739-8528, JapanInstitute for Advanced Biosciences, Keio University, Tsuruoka 997-0052, JapanAmano Enzyme Inc., Nagoya 460-8630, JapanAmano Enzyme Inc., Nagoya 460-8630, Japan<i>Aspergillus</i>-derived protease and lipase, which are involved in the production of <i>Aspergillus</i>-fermented foods, are consumed as digestive enzyme supplements. A marked bifidogenic effect of supplemental <i>Aspergillus</i> protease preparation (AP) in rats fed with a high-fat diet was identified. This study was conducted to examine whether the consumption of <i>Aspergillus</i>-derived lipase exerts similar bifidogenic effect. Rats were fed diets supplemented with either an <i>Aspergillus</i>-derived lipase preparation (AL) or AP at 0.1% for two weeks. 16S rRNA gene sequencing analysis indicated that supplemental AL and AP markedly influenced cecal microbial community. At the phylum level, treatment with AL and AP resulted in a lower relative abundance of Firmicutes and Bacteroidetes, but a higher relative abundance of Actinobacteria and Proteobacteria than the control rats (<i>p</i> < 0.05). At the genus level, AL and AP remarkedly elevated the relative abundances of <i>Bifidobacterium</i>, <i>Collinsella</i>, and <i>Enterococcus</i>, but significantly reduced those of <i>Oscillospira</i>, <i>Dorea</i>, and <i>Coprobacillus</i> (<i>p</i> < 0.05). These modulations were similar to those reported by several studies with typical prebiotic oligosaccharides. Notably, the bifidogenic effect of AL was much greater than that of AP. Our results show that the two different <i>Aspergillus</i>-derived preparations, AL and AP, have strong bifidogenic effects and can change the microbiota’s composition.https://www.mdpi.com/2311-5637/7/4/294<i>Aspergillus</i>lipaseproteasebifidogenic effectprebiotics16S rRNA gene sequencing |
| spellingShingle | Yongshou Yang Thanutchaporn Kumrungsee Norihisa Kato Shinji Fukuda Manabu Kuroda Shotaro Yamaguchi Supplemental <i>Aspergillus</i> Lipase and Protease Preparations Display Powerful Bifidogenic Effects and Modulate the Gut Microbiota Community of Rats Fermentation <i>Aspergillus</i> lipase protease bifidogenic effect prebiotics 16S rRNA gene sequencing |
| title | Supplemental <i>Aspergillus</i> Lipase and Protease Preparations Display Powerful Bifidogenic Effects and Modulate the Gut Microbiota Community of Rats |
| title_full | Supplemental <i>Aspergillus</i> Lipase and Protease Preparations Display Powerful Bifidogenic Effects and Modulate the Gut Microbiota Community of Rats |
| title_fullStr | Supplemental <i>Aspergillus</i> Lipase and Protease Preparations Display Powerful Bifidogenic Effects and Modulate the Gut Microbiota Community of Rats |
| title_full_unstemmed | Supplemental <i>Aspergillus</i> Lipase and Protease Preparations Display Powerful Bifidogenic Effects and Modulate the Gut Microbiota Community of Rats |
| title_short | Supplemental <i>Aspergillus</i> Lipase and Protease Preparations Display Powerful Bifidogenic Effects and Modulate the Gut Microbiota Community of Rats |
| title_sort | supplemental i aspergillus i lipase and protease preparations display powerful bifidogenic effects and modulate the gut microbiota community of rats |
| topic | <i>Aspergillus</i> lipase protease bifidogenic effect prebiotics 16S rRNA gene sequencing |
| url | https://www.mdpi.com/2311-5637/7/4/294 |
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