Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space Station
The establishment of steady-state continuous crop production during long-term deep space missions is critical for providing consistent nutritional and psychological benefits for the crew, potentially improving their health and performance. Three technology demonstrations were completed achieving sim...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/11/10/1060 |
| _version_ | 1797514133273313280 |
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| author | Mary E. Hummerick Christina L. M. Khodadad Anirudha R. Dixit Lashelle E. Spencer Gretchen J. Maldonado-Vasquez Jennifer L. Gooden Cory J. Spern Jason A. Fischer Nicole Dufour Raymond M. Wheeler Matthew W. Romeyn Trent M. Smith Gioia D. Massa Ye Zhang |
| author_facet | Mary E. Hummerick Christina L. M. Khodadad Anirudha R. Dixit Lashelle E. Spencer Gretchen J. Maldonado-Vasquez Jennifer L. Gooden Cory J. Spern Jason A. Fischer Nicole Dufour Raymond M. Wheeler Matthew W. Romeyn Trent M. Smith Gioia D. Massa Ye Zhang |
| author_sort | Mary E. Hummerick |
| collection | DOAJ |
| description | The establishment of steady-state continuous crop production during long-term deep space missions is critical for providing consistent nutritional and psychological benefits for the crew, potentially improving their health and performance. Three technology demonstrations were completed achieving simultaneous multi-species plant growth and the concurrent use of two Veggie units on the International Space Station (ISS). Microbiological characterization using molecular and culture-based methods was performed on leaves and roots from two harvests of three leafy greens, red romaine lettuce (<i>Lactuca sativa</i> cv. ‘Outredgeous’); mizuna mustard, (<i>Brassica rapa</i> var <i>japonica</i>); and green leaf lettuce, (<i>Lactuca sativa</i> cv. Waldmann’s) and associated rooting pillow components and Veggie chamber surfaces. Culture based enumeration and pathogen screening indicated the leafy greens were safe for consumption. Surface samples of the Veggie facility and plant pillows revealed low counts of bacteria and fungi and are commonly isolated on ISS. Community analysis was completed with 16S rRNA amplicon sequencing. Comparisons between pillow components, and plant tissue types from VEG-03D, E, and F revealed higher diversity in roots and rooting substrate than the leaves and wick. This work provides valuable information for food production-related research on the ISS and the impact of the plant microbiome on this unique closed environment. |
| first_indexed | 2024-03-10T06:27:19Z |
| format | Article |
| id | doaj.art-cb6011f3092549bfa90a98f690329bd6 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T06:27:19Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-cb6011f3092549bfa90a98f690329bd62023-11-22T18:52:55ZengMDPI AGLife2075-17292021-10-011110106010.3390/life11101060Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space StationMary E. Hummerick0Christina L. M. Khodadad1Anirudha R. Dixit2Lashelle E. Spencer3Gretchen J. Maldonado-Vasquez4Jennifer L. Gooden5Cory J. Spern6Jason A. Fischer7Nicole Dufour8Raymond M. Wheeler9Matthew W. Romeyn10Trent M. Smith11Gioia D. Massa12Ye Zhang13Kennedy Space Center, Amentum Services, Inc., LASSO, Merritt Island, FL 32899, USAKennedy Space Center, Amentum Services, Inc., LASSO, Merritt Island, FL 32899, USAKennedy Space Center, Amentum Services, Inc., LASSO, Merritt Island, FL 32899, USAKennedy Space Center, Amentum Services, Inc., LASSO, Merritt Island, FL 32899, USAKennedy Space Center, Amentum Services, Inc., LASSO, Merritt Island, FL 32899, USAKennedy Space Center, Amentum Services, Inc., LASSO, Merritt Island, FL 32899, USAKennedy Space Center, Amentum Services, Inc., LASSO, Merritt Island, FL 32899, USAKennedy Space Center, Amentum Services, Inc., LASSO, Merritt Island, FL 32899, USAKennedy Space Center, Utilization and Life Sciences Office, NASA, Merritt Island, FL 32899, USAKennedy Space Center, Utilization and Life Sciences Office, NASA, Merritt Island, FL 32899, USAKennedy Space Center, Utilization and Life Sciences Office, NASA, Merritt Island, FL 32899, USAKennedy Space Center, Utilization and Life Sciences Office, NASA, Merritt Island, FL 32899, USAKennedy Space Center, Utilization and Life Sciences Office, NASA, Merritt Island, FL 32899, USAKennedy Space Center, Utilization and Life Sciences Office, NASA, Merritt Island, FL 32899, USAThe establishment of steady-state continuous crop production during long-term deep space missions is critical for providing consistent nutritional and psychological benefits for the crew, potentially improving their health and performance. Three technology demonstrations were completed achieving simultaneous multi-species plant growth and the concurrent use of two Veggie units on the International Space Station (ISS). Microbiological characterization using molecular and culture-based methods was performed on leaves and roots from two harvests of three leafy greens, red romaine lettuce (<i>Lactuca sativa</i> cv. ‘Outredgeous’); mizuna mustard, (<i>Brassica rapa</i> var <i>japonica</i>); and green leaf lettuce, (<i>Lactuca sativa</i> cv. Waldmann’s) and associated rooting pillow components and Veggie chamber surfaces. Culture based enumeration and pathogen screening indicated the leafy greens were safe for consumption. Surface samples of the Veggie facility and plant pillows revealed low counts of bacteria and fungi and are commonly isolated on ISS. Community analysis was completed with 16S rRNA amplicon sequencing. Comparisons between pillow components, and plant tissue types from VEG-03D, E, and F revealed higher diversity in roots and rooting substrate than the leaves and wick. This work provides valuable information for food production-related research on the ISS and the impact of the plant microbiome on this unique closed environment.https://www.mdpi.com/2075-1729/11/10/1060veggieISSmicrobiomelettucemizunaphyllosphere |
| spellingShingle | Mary E. Hummerick Christina L. M. Khodadad Anirudha R. Dixit Lashelle E. Spencer Gretchen J. Maldonado-Vasquez Jennifer L. Gooden Cory J. Spern Jason A. Fischer Nicole Dufour Raymond M. Wheeler Matthew W. Romeyn Trent M. Smith Gioia D. Massa Ye Zhang Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space Station Life veggie ISS microbiome lettuce mizuna phyllosphere |
| title | Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space Station |
| title_full | Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space Station |
| title_fullStr | Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space Station |
| title_full_unstemmed | Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space Station |
| title_short | Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space Station |
| title_sort | spatial characterization of microbial communities on multi species leafy greens grown simultaneously in the vegetable production systems on the international space station |
| topic | veggie ISS microbiome lettuce mizuna phyllosphere |
| url | https://www.mdpi.com/2075-1729/11/10/1060 |
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