Evaluation and Comparison of Genomic DNA Extraction Methods and PCR Optimization on Archival Formalin-Fixed and Paraffin-Embedded Tissues of Oral Squamous Cell Carcinoma

Evaluation and Comparison of Genomic DNA Extraction Methods and PCR Optimization on Archival Formalin-Fixed and Paraffin-Embedded Tissues of Oral Squamous Cell Carcinoma

Recovery and amplification of nucleic acids from archived formalin-fixed tissue samples is the most developing field in retrospective genetic studies. We compared different deparaffinization methods and DNA isolation techniques, and intergroup comparisons were performed to evaluate the effectiveness...

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Bibliographic Details
Main Authors: Samar Saeed Khan, Manisha Tijare, Sowmya Kasetty, Megha Jain, Ahmed Alamoudi, Hammam Ahmed Bahammam, Sarah Ahmed Bahammam, Maha A. Bahammam, Saranya Varadarajan, A. Thirumal Raj, Shankargouda Patil
Format: Article
Language:English
Published: MDPI AG 2022-05-01
Series:Diagnostics
Subjects:
oral squamous cell carcinoma
formalin-fixed tissues
formalin-fixed paraffin-embedded tissues
deparaffinization
polymerase chain reaction
Online Access:https://www.mdpi.com/2075-4418/12/5/1219

Internet

https://www.mdpi.com/2075-4418/12/5/1219

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