Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W

Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W

UBE2W catalyzes the ubiquitination of protein N-termini but its substrate spectrum is largely unknown. Here, the authors discover mAbs selective for peptides derived from N-terminally ubiquitinated proteins, solve the structure of a peptide-bound mAb and apply the mAbs to map endogenous UBE2W substr...

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Bibliographic Details
Main Authors: Christopher W. Davies, Simon E. Vidal, Lilian Phu, Jawahar Sudhamsu, Trent B. Hinkle, Scott Chan Rosenberg, Frances-Rose Schumacher, Yi Jimmy Zeng, Carsten Schwerdtfeger, Andrew S. Peterson, Jennie R. Lill, Christopher M. Rose, Andrey S. Shaw, Ingrid E. Wertz, Donald S. Kirkpatrick, James T. Koerber
Format: Article
Language:English
Published: Nature Portfolio 2021-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-24669-6

Internet

https://doi.org/10.1038/s41467-021-24669-6

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