Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
UBE2W catalyzes the ubiquitination of protein N-termini but its substrate spectrum is largely unknown. Here, the authors discover mAbs selective for peptides derived from N-terminally ubiquitinated proteins, solve the structure of a peptide-bound mAb and apply the mAbs to map endogenous UBE2W substr...
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Bibliographic Details
| Main Authors: |
Christopher W. Davies,
Simon E. Vidal,
Lilian Phu,
Jawahar Sudhamsu,
Trent B. Hinkle,
Scott Chan Rosenberg,
Frances-Rose Schumacher,
Yi Jimmy Zeng,
Carsten Schwerdtfeger,
Andrew S. Peterson,
Jennie R. Lill,
Christopher M. Rose,
Andrey S. Shaw,
Ingrid E. Wertz,
Donald S. Kirkpatrick,
James T. Koerber |
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| Format: | Article
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| Language: | English |
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| Published: |
Nature Portfolio
2021-07-01
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| Series: | Nature Communications
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| Online Access: | https://doi.org/10.1038/s41467-021-24669-6
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