Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
UBE2W catalyzes the ubiquitination of protein N-termini but its substrate spectrum is largely unknown. Here, the authors discover mAbs selective for peptides derived from N-terminally ubiquitinated proteins, solve the structure of a peptide-bound mAb and apply the mAbs to map endogenous UBE2W substr...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-24669-6 |
| _version_ | 1818682429160816640 |
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| author | Christopher W. Davies Simon E. Vidal Lilian Phu Jawahar Sudhamsu Trent B. Hinkle Scott Chan Rosenberg Frances-Rose Schumacher Yi Jimmy Zeng Carsten Schwerdtfeger Andrew S. Peterson Jennie R. Lill Christopher M. Rose Andrey S. Shaw Ingrid E. Wertz Donald S. Kirkpatrick James T. Koerber |
| author_facet | Christopher W. Davies Simon E. Vidal Lilian Phu Jawahar Sudhamsu Trent B. Hinkle Scott Chan Rosenberg Frances-Rose Schumacher Yi Jimmy Zeng Carsten Schwerdtfeger Andrew S. Peterson Jennie R. Lill Christopher M. Rose Andrey S. Shaw Ingrid E. Wertz Donald S. Kirkpatrick James T. Koerber |
| author_sort | Christopher W. Davies |
| collection | DOAJ |
| description | UBE2W catalyzes the ubiquitination of protein N-termini but its substrate spectrum is largely unknown. Here, the authors discover mAbs selective for peptides derived from N-terminally ubiquitinated proteins, solve the structure of a peptide-bound mAb and apply the mAbs to map endogenous UBE2W substrates by proteomics. |
| first_indexed | 2024-12-17T10:18:42Z |
| format | Article |
| id | doaj.art-cbfb2674260f4b51a1c495c83e07dcb5 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-17T10:18:42Z |
| publishDate | 2021-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-cbfb2674260f4b51a1c495c83e07dcb52022-12-21T21:52:51ZengNature PortfolioNature Communications2041-17232021-07-0112111910.1038/s41467-021-24669-6Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2WChristopher W. Davies0Simon E. Vidal1Lilian Phu2Jawahar Sudhamsu3Trent B. Hinkle4Scott Chan Rosenberg5Frances-Rose Schumacher6Yi Jimmy Zeng7Carsten Schwerdtfeger8Andrew S. Peterson9Jennie R. Lill10Christopher M. Rose11Andrey S. Shaw12Ingrid E. Wertz13Donald S. Kirkpatrick14James T. Koerber15Department of Antibody Engineering, Genentech, Inc.Departments of Molecular Oncology and Early Discovery Biochemistry, Genentech, Inc.Department of Microchemistry, Proteomics, and Lipidomics, Genentech, Inc.Department of Structural Biology, Genentech, Inc.Department of Microchemistry, Proteomics, and Lipidomics, Genentech, Inc.Departments of Molecular Oncology and Early Discovery Biochemistry, Genentech, Inc.Department of Microchemistry, Proteomics, and Lipidomics, Genentech, Inc.Department of Microchemistry, Proteomics, and Lipidomics, Genentech, Inc.Boston Biochem, a Bio-Techne Brand 840 Memorial DriveDepartment of Molecular Biology, Genentech, Inc.Department of Microchemistry, Proteomics, and Lipidomics, Genentech, Inc.Department of Microchemistry, Proteomics, and Lipidomics, Genentech, Inc.Research Biology, Genentech, Inc.Departments of Molecular Oncology and Early Discovery Biochemistry, Genentech, Inc.Department of Microchemistry, Proteomics, and Lipidomics, Genentech, Inc.Department of Antibody Engineering, Genentech, Inc.UBE2W catalyzes the ubiquitination of protein N-termini but its substrate spectrum is largely unknown. Here, the authors discover mAbs selective for peptides derived from N-terminally ubiquitinated proteins, solve the structure of a peptide-bound mAb and apply the mAbs to map endogenous UBE2W substrates by proteomics.https://doi.org/10.1038/s41467-021-24669-6 |
| spellingShingle | Christopher W. Davies Simon E. Vidal Lilian Phu Jawahar Sudhamsu Trent B. Hinkle Scott Chan Rosenberg Frances-Rose Schumacher Yi Jimmy Zeng Carsten Schwerdtfeger Andrew S. Peterson Jennie R. Lill Christopher M. Rose Andrey S. Shaw Ingrid E. Wertz Donald S. Kirkpatrick James T. Koerber Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W Nature Communications |
| title | Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W |
| title_full | Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W |
| title_fullStr | Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W |
| title_full_unstemmed | Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W |
| title_short | Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W |
| title_sort | antibody toolkit reveals n terminally ubiquitinated substrates of ube2w |
| url | https://doi.org/10.1038/s41467-021-24669-6 |
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