Protocol for in vitro assessment of human monocyte transendothelial migration using a high-throughput live cell imaging system

Summary: In vitro modeling of the different steps of immune cell recruitment is essential to decipher the role of endothelial cells in this process. Here, we present a protocol for the assessment of human monocyte transendothelial migration using a live cell imaging system. We describe steps for cul...

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Bibliographic Details
Main Authors: Ségolène Ladaigue, Vincent Paget, Anne-Charlotte Lefranc, Monica Quitoco, Emilie Bacquer, Fabien Milliat, Olivier Guipaud
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723003556